Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/9772
Title
Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus
Author(s)
Jiménez-Zaragoza, Manuel | Yubero, Marina Pl | Martin-Forero, Esther ISCIII | Castón, Jose R | Reguera, David | Luque, Daniel ISCIII | de Pablo, Pedro J | Rodriguez Martinez, Javier M ISCIII
Date issued
2018
Citation
Elife. 2018 Sep 11;7. pii: e37295.
Language
Inglés
Document type
journal article
Abstract
The functions performed by the concentric shells of multilayered dsRNA viruses require specific protein interactions that can be directly explored through their mechanical properties. We studied the stiffness, breaking force, critical strain and mechanical fatigue of individual Triple, Double and Single layered rotavirus (RV) particles. Our results, in combination with Finite Element simulations, demonstrate that the mechanics of the external layer provides the resistance needed to counteract the stringent conditions of extracellular media. Our experiments, in combination with electrostatic analyses, reveal a strong interaction between the two outer layers and how it is suppressed by the removal of calcium ions, a key step for transcription initiation. The intermediate layer presents weak hydrophobic interactions with the inner layer that allow the assembly and favor the conformational dynamics needed for transcription. Our work shows how the biophysical properties of the three shells are finely tuned to produce an infective RV virion.
Subject
Atomic force microscopy | Fatigue | Nanoindentation | Physical virology | Physics of living systems | Virus
MESH
Finite Element Analysis | Microscopy, Atomic Force | Models, Biological | Nanoparticles | Rotavirus | Viral Proteins | Virion | Biophysical Phenomena
Online version
DOI
Collections