2024-03-28T16:53:59Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/97722023-10-09T11:22:31Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
Repisalud
author
Jiménez-Zaragoza, Manuel
author
Yubero, Marina Pl
author
Martin-Forero, Esther
author
Castón, Jose R
author
Reguera, David
author
Luque, Daniel
author
de Pablo, Pedro J
author
Rodriguez Martinez, Javier M
funder
Ministerio de Economía y Competitividad (España)
funder
Comunidad de Madrid (España)
2020-04-28T08:29:24Z
2020-04-28T08:29:24Z
2018
Elife. 2018 Sep 11;7. pii: e37295.
2050-084X
http://hdl.handle.net/20.500.12105/9772
30201094
10.7554/eLife.37295
2050-084X
eLife
The functions performed by the concentric shells of multilayered dsRNA viruses require specific protein interactions that can be directly explored through their mechanical properties. We studied the stiffness, breaking force, critical strain and mechanical fatigue of individual Triple, Double and Single layered rotavirus (RV) particles. Our results, in combination with Finite Element simulations, demonstrate that the mechanics of the external layer provides the resistance needed to counteract the stringent conditions of extracellular media. Our experiments, in combination with electrostatic analyses, reveal a strong interaction between the two outer layers and how it is suppressed by the removal of calcium ions, a key step for transcription initiation. The intermediate layer presents weak hydrophobic interactions with the inner layer that allow the assembly and favor the conformational dynamics needed for transcription. Our work shows how the biophysical properties of the three shells are finely tuned to produce an infective RV virion.
eng
Atomic force microscopy
Fatigue
Nanoindentation
Physical virology
Physics of living systems
Virus
Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus
journal article
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/9772/1/BiophysicalPropertiesOfSingle_2018.pdf
File
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BiophysicalPropertiesOfSingle_2018.pdf
URL
https://repisalud.isciii.es/bitstream/20.500.12105/9772/3/BiophysicalPropertiesOfSingle_2018.pdf.txt
File
MD5
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BiophysicalPropertiesOfSingle_2018.pdf.txt