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dc.contributor.authorJiménez-Zaragoza, Manuel
dc.contributor.authorYubero, Marina Pl
dc.contributor.authorMartín-Forero, Esther 
dc.contributor.authorCastón, Jose R
dc.contributor.authorReguera, David
dc.contributor.authorLuque, Daniel 
dc.contributor.authorde Pablo, Pedro J
dc.contributor.authorRodriguez Martinez, Javier M 
dc.date.accessioned2020-04-28T08:29:24Z
dc.date.available2020-04-28T08:29:24Z
dc.date.issued2018
dc.identifier.citationElife. 2018 Sep 11;7. pii: e37295.es_ES
dc.identifier.issn2050-084Xes_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9772
dc.description.abstractThe functions performed by the concentric shells of multilayered dsRNA viruses require specific protein interactions that can be directly explored through their mechanical properties. We studied the stiffness, breaking force, critical strain and mechanical fatigue of individual Triple, Double and Single layered rotavirus (RV) particles. Our results, in combination with Finite Element simulations, demonstrate that the mechanics of the external layer provides the resistance needed to counteract the stringent conditions of extracellular media. Our experiments, in combination with electrostatic analyses, reveal a strong interaction between the two outer layers and how it is suppressed by the removal of calcium ions, a key step for transcription initiation. The intermediate layer presents weak hydrophobic interactions with the inner layer that allow the assembly and favor the conformational dynamics needed for transcription. Our work shows how the biophysical properties of the three shells are finely tuned to produce an infective RV virion.es_ES
dc.description.sponsorshipThis work was supported by grants from the Spanish Ministry of Economy and Competitivity FIS2014-59562-R, FIS2017-89549-R and ‘María de Maeztu’ Program for Units of Excellence in R and D (MDM-2014–0377) to PJP, BFU2013-43149-R to DL and JMR, FIS2015- 67837 P to DR, FIS2015-71108-REDT to PJP, DL, DR, and JMR, and BFU2014-55475-R (Spanish Ministry of Economy and Competitivity) and S2013/MIT-2807 (Comunidad Autónoma de Madrid) to JRC.es_ES
dc.language.isoenges_ES
dc.publishereLife Sciences Publicationses_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectatomic force microscopyes_ES
dc.subjectfatiguees_ES
dc.subjectnanoindentationes_ES
dc.subjectphysical virologyes_ES
dc.subjectphysics of living systemses_ES
dc.subjectviruses_ES
dc.subject.meshFinite Element Analysis es_ES
dc.subject.meshMicroscopy, Atomic Force es_ES
dc.subject.meshModels, Biologicales_ES
dc.subject.meshNanoparticles es_ES
dc.subject.meshRotavirus es_ES
dc.subject.meshViral Proteins es_ES
dc.subject.meshVirion es_ES
dc.subject.meshBiophysical Phenomena es_ES
dc.titleBiophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered viruses_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID30201094es_ES
dc.format.volume7es_ES
dc.identifier.doi10.7554/eLife.37295es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderComunidad de Madrides_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn2050-084Xes_ES
dc.relation.publisherversionhttps://doi.org/10.7554/eLife.37295es_ES
dc.identifier.journaleLifees_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/FIS20 14-59562-Res_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/FIS2017-89549-Res_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/MDM-2014–0377es_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/BFU2013-43149-Res_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/FIS2015- 67837 Pes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/FIS2015-71108-REDTes_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/BFU2014-55475-Res_ES
dc.relation.projectIDinfo:eu_repo/grantAgreement/ES/S2013/MIT-2807es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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