Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/8888
HCV-coinfection is related to an increased HIV-1 reservoir size in cART-treated HIV patients: a cross-sectional study
Lopez-Huertas, Maria Rosa ISCIII | Palladino, Claudia | Garrido-Arquero, Marta ISCIII | Esteban-Cartelle, Beatriz ISCIII | Sanchez-Carrillo, Marta ISCIII | Martinez-Roman, Paula ISCIII | Martín-Carbonero, Luz | Ryan, Pablo | Domínguez-Domínguez, Lourdes | Santos, Ignacio De Los | Moral, Sara De La Fuente | Benito, José Miguel | Rallón, Norma | Alcamí, José ISCIII | Resino, Salvador ISCIII | Fernandez-Rodriguez, Amanda ISCIII | Coiras, Mayte ISCIII | Briz, Verónica ISCIII
Sci Rep. 2019 Apr 3;9(1):5606.
In HIV-1/HCV-coinfected patients, chronic HCV infection leads to an increased T-lymphocyte immune activation compared to HIV-monoinfected patients, thereby likely contributing to increase HIV-1 reservoir that is the major barrier for its eradication. Our objective was to evaluate the influence of HCV coinfection in HIV-1 viral reservoir size in resting (r) CD4+ T-cells (CD25-CD69-HLADR-). Multicenter cross-sectional study of 97 cART-treated HIV-1 patients, including 36 patients with HIV and HCV-chronic co-infection without anti-HCV treatment, 32 HIV patients with HCV spontaneous clearance and 29 HIV-monoinfected patients. rCD4+ T-cells were isolated and total DNA was extracted. HIV viral reservoir was measured by Alu-LTR qPCR. Differences between groups were calculated with a generalized linear model. Overall, 63.9% were men, median age of 41 years and Caucasian. Median CD4+ and CD8+ T-lymphocytes were 725 and 858 cells/mm3, respectively. CD4+ T nadir cells was 305 cells/mm3. Proviral HIV-1 DNA size was significantly increased in chronic HIV/HCV-coinfected compared to HIV-monoinfected patients (206.21 ± 47.38 vs. 87.34 ± 22.46, respectively; P = 0.009), as well as in spontaneously clarified HCV co-infected patients when compared to HIV-monoinfected individuals (136.20 ± 33.20; P = 0.009). HIV-1/HCV co-infected patients showed a larger HIV-1 reservoir size in comparison to HIV-monoinfected individuals. This increase could lead to a greater complexity in the elimination of HIV-1 reservoir in HIV-1/HCV-coinfected individuals, which should be considered in the current strategies for the elimination of HIV-1 reservoir.
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