2024-03-29T10:36:18Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/88882022-10-06T08:13:52Zcom_20.500.12105_15322com_20.500.12105_2051com_20.500.12105_2060com_20.500.12105_2052col_20.500.12105_16988col_20.500.12105_16977col_20.500.12105_16963col_20.500.12105_16938col_20.500.12105_2061
00925njm 22002777a 4500
dc
Lopez-Huertas, Maria Rosa
author
Palladino, Claudia
author
Garrido-Arquero, Marta
author
Esteban-Cartelle, Beatriz
author
Sanchez-Carrillo, Marta
author
Martinez-Roman, Paula
author
Martín-Carbonero, Luz
author
Ryan, Pablo
author
Domínguez-Domínguez, Lourdes
author
De Los Santos, Ignacio
author
De La Fuente Moral, Sara
author
Benito, José Miguel
author
Rallón, Norma
author
Alcamí, José
author
Resino, Salvador
author
Fernandez-Rodriguez, Amanda
author
Coiras, Mayte
author
Briz, Veronica
author
2019-04-03
In HIV-1/HCV-coinfected patients, chronic HCV infection leads to an increased T-lymphocyte immune activation compared to HIV-monoinfected patients, thereby likely contributing to increase HIV-1 reservoir that is the major barrier for its eradication. Our objective was to evaluate the influence of HCV coinfection in HIV-1 viral reservoir size in resting (r) CD4+ T-cells (CD25-CD69-HLADR-). Multicenter cross-sectional study of 97 cART-treated HIV-1 patients, including 36 patients with HIV and HCV-chronic co-infection without anti-HCV treatment, 32 HIV patients with HCV spontaneous clearance and 29 HIV-monoinfected patients. rCD4+ T-cells were isolated and total DNA was extracted. HIV viral reservoir was measured by Alu-LTR qPCR. Differences between groups were calculated with a generalized linear model. Overall, 63.9% were men, median age of 41 years and Caucasian. Median CD4+ and CD8+ T-lymphocytes were 725 and 858 cells/mm3, respectively. CD4+ T nadir cells was 305 cells/mm3. Proviral HIV-1 DNA size was significantly increased in chronic HIV/HCV-coinfected compared to HIV-monoinfected patients (206.21 ± 47.38 vs. 87.34 ± 22.46, respectively; P = 0.009), as well as in spontaneously clarified HCV co-infected patients when compared to HIV-monoinfected individuals (136.20 ± 33.20; P = 0.009). HIV-1/HCV co-infected patients showed a larger HIV-1 reservoir size in comparison to HIV-monoinfected individuals. This increase could lead to a greater complexity in the elimination of HIV-1 reservoir in HIV-1/HCV-coinfected individuals, which should be considered in the current strategies for the elimination of HIV-1 reservoir.
Sci Rep. 2019 Apr 3;9(1):5606.
2045-2322
http://hdl.handle.net/20.500.12105/8888
30944340
10.1038/s41598-019-41788-9
2045-2322
Scientific reports
HCV-coinfection is related to an increased HIV-1 reservoir size in cART-treated HIV patients: a cross-sectional study