Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/7948
Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root
Andres-Manzano, Maria J. CNIC | Andres, Vicente CNIC | Dorado, Beatriz CNIC
Methods Mol Biol. 2015; 1339:85-99
Methods in Molelcular Biology
Methods for staining tissues with Oil Red O and hematoxylin-eosin are classical histological techniques that are widely used to quantify atherosclerotic burden in mouse tissues because of their ease of use, reliability, and the large amount of information they provide. These stains can provide quantitative data about the impact of a genetic or environmental factor on atherosclerotic burden and on the initiation, progression, or regression of the disease, and can also be used to evaluate the efficacy of drugs designed to prevent or treat atherosclerosis. This chapter provides protocols for quantifying atherosclerotic burden in mouse aorta and aortic root, including methods for dissection, Oil Red O staining, hematoxylin-eosin staining, and image analysis.
Animals | Aorta | Aortic Diseases | Atherosclerosis | Disease Models, Animal | Dissection | Image Interpretation, Computer-Assisted | Mice | Microscopy | Paraffin Embedding | Plaque, Atherosclerotic | Severity of Illness Index | Staining and Labeling | Azo Compounds | Coloring Agents | Eosine Yellowish-(YS) | Hematoxylin
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