2024-03-29T06:39:13Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/79482022-10-25T10:17:33Zcom_20.500.12105_2145com_20.500.12105_2051com_20.500.12105_2144col_20.500.12105_2149
00925njm 22002777a 4500
dc
Andres-Manzano, Maria J.
author
Andres, Vicente
author
Dorado, Beatriz
author
2015
Methods for staining tissues with Oil Red O and hematoxylin-eosin are classical histological techniques that are widely used to quantify atherosclerotic burden in mouse tissues because of their ease of use, reliability, and the large amount of information they provide. These stains can provide quantitative data about the impact of a genetic or environmental factor on atherosclerotic burden and on the initiation, progression, or regression of the disease, and can also be used to evaluate the efficacy of drugs designed to prevent or treat atherosclerosis. This chapter provides protocols for quantifying atherosclerotic burden in mouse aorta and aortic root, including methods for dissection, Oil Red O staining, hematoxylin-eosin staining, and image analysis.
Methods Mol Biol. 2015; 1339:85-99
978-1-4939-2929-0
1064-3745
http://hdl.handle.net/20.500.12105/7948
26445782
10.1007/978-1-4939-2929-0_5
1940-6029
Methods in molecular biology (Clifton, N.J.)
Aorta
Aortic root
Atherosclerosis
Dissection
Hematoxylin-eosin
Oil Red O
Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root