2024-03-28T17:32:31Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/79482022-10-25T10:17:33Zcom_20.500.12105_2145com_20.500.12105_2051com_20.500.12105_2144col_20.500.12105_2149
Repisalud
author
Andres-Manzano, Maria J.
author
Andres, Vicente
author
Dorado, Beatriz
funder
Ministerio de Economía y Competitividad (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Unión Europea. Comisión Europea
funder
Progeria Research Foundation
funder
Fundación ProCNIC
2019-07-19T05:57:31Z
2019-07-19T05:57:31Z
2015
Methods Mol Biol. 2015; 1339:85-99
978-1-4939-2929-0
1064-3745
http://hdl.handle.net/20.500.12105/7948
26445782
10.1007/978-1-4939-2929-0_5
1940-6029
Methods in molecular biology (Clifton, N.J.)
Methods for staining tissues with Oil Red O and hematoxylin-eosin are classical histological techniques that are widely used to quantify atherosclerotic burden in mouse tissues because of their ease of use, reliability, and the large amount of information they provide. These stains can provide quantitative data about the impact of a genetic or environmental factor on atherosclerotic burden and on the initiation, progression, or regression of the disease, and can also be used to evaluate the efficacy of drugs designed to prevent or treat atherosclerosis. This chapter provides protocols for quantifying atherosclerotic burden in mouse aorta and aortic root, including methods for dissection, Oil Red O staining, hematoxylin-eosin staining, and image analysis.
eng
Aorta
Aortic root
Atherosclerosis
Dissection
Hematoxylin-eosin
Oil Red O
Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root
book part
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URL
https://repisalud.isciii.es/bitstream/20.500.12105/7948/1/OilRedOHematoxylin_2015.pdf
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https://repisalud.isciii.es/bitstream/20.500.12105/7948/3/OilRedOHematoxylin_2015.pdf.txt
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OilRedOHematoxylin_2015.pdf.txt