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dc.contributor.authorAndres-Manzano, Maria J. 
dc.contributor.authorAndres, Vicente 
dc.contributor.authorDorado, Beatriz 
dc.date.accessioned2019-07-19T05:57:31Z
dc.date.available2019-07-19T05:57:31Z
dc.date.issued2015
dc.identifier.citationMethods Mol Biol. 2015; 1339:85-99es_ES
dc.identifier.isbn978-1-4939-2929-0es_ES
dc.identifier.issn1064-3745
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7948
dc.description.abstractMethods for staining tissues with Oil Red O and hematoxylin-eosin are classical histological techniques that are widely used to quantify atherosclerotic burden in mouse tissues because of their ease of use, reliability, and the large amount of information they provide. These stains can provide quantitative data about the impact of a genetic or environmental factor on atherosclerotic burden and on the initiation, progression, or regression of the disease, and can also be used to evaluate the efficacy of drugs designed to prevent or treat atherosclerosis. This chapter provides protocols for quantifying atherosclerotic burden in mouse aorta and aortic root, including methods for dissection, Oil Red O staining, hematoxylin-eosin staining, and image analysis.es_ES
dc.description.sponsorshipThe authors thank Simon Bartlett for editorial assistance. Work in VA’s laboratory is supported by grants SAF2013-46663-R and RD12/0042/0028 from the Spanish Ministerio de Economía y Competitividad (MINECO) with co-funding from the Fondo Europeo de Desarrollo Regional (FEDER), the European Commission (Liphos, Grant Agreement No. 317916), and the Progeria Research Foundation (Established Investigator Award). The CNIC is supported by the MINECO and the Pro-CNIC Foundation.es_ES
dc.language.isoenges_ES
dc.publisherSpringeres_ES
dc.relation.ispartofseriesMethods in Molelcular Biologyes_ES
dc.relation.isversionofPostprintes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAortaes_ES
dc.subjectAortic rootes_ES
dc.subjectAtherosclerosises_ES
dc.subjectDissectiones_ES
dc.subjectHematoxylin-eosines_ES
dc.subjectOil Red Oes_ES
dc.subject.meshAnimals es_ES
dc.subject.meshAorta es_ES
dc.subject.meshAortic Diseases es_ES
dc.subject.meshAtherosclerosis es_ES
dc.subject.meshDisease Models, Animales_ES
dc.subject.meshDissection es_ES
dc.subject.meshImage Interpretation, Computer-Assisted es_ES
dc.subject.meshMice es_ES
dc.subject.meshMicroscopy es_ES
dc.subject.meshParaffin Embedding es_ES
dc.subject.meshPlaque, Atherosclerotices_ES
dc.subject.meshSeverity of Illness Index es_ES
dc.subject.meshStaining and Labeling es_ES
dc.subject.meshAzo Compounds es_ES
dc.subject.meshColoring Agents es_ES
dc.subject.meshEosine Yellowish-(YS) es_ES
dc.subject.meshHematoxylin es_ES
dc.titleOil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Rootes_ES
dc.typeCapítulo de libroes_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.identifier.pubmedID26445782
dc.format.volume1339
dc.format.page99
dc.identifier.doi10.1007/978-1-4939-2929-0_5es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderEuropean Regional Development Fund (ERDF/FEDER)es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderProgeria Research Foundationes_ES
dc.contributor.funderFundación ProCNICes_ES
dc.identifier.e-issn1940-6029
dc.relation.ispartofseriesnumber1339es_ES
dc.relation.publisherversionhttps://doi.org/10.1007/978-1-4939-2929-0_5es_ES
dc.identifier.journalMethods in molecular biology (Clifton, N.J.)
dc.repisalud.orgCNICCNIC::Grupos de investigación::Fisiopatología Cardiovascular Molecular y Genéticaes_ES
dc.repisalud.institucionCNICes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2013-46663-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RD12/0042/0028es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/317916/EUes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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