Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/19959
Title
Enhancing Hepatic Fatty Acid Oxidation as a Strategy for Reversing Metabolic Disorders Programmed by Maternal Undernutrition During Gestation
Author(s)
Date issued
2014
Citation
Maria Torrens J, Orellana-Gavalda JM, Palou March M, Sánchez J, Herrero L, Pico C, et al. Enhancing Hepatic Fatty Acid Oxidation as a Strategy for Reversing Metabolic Disorders Programmed by Maternal Undernutrition During Gestation. Cell Physiol Biochem. 2014;33(5):1498-515. Epub 2014 May 14.
Language
Inglés
Document type
research article
Abstract
Background/Aims: Moderate maternal calorie-restriction during gestation programmes offspring for a major propensity to develop metabolic alterations in adulthood. We aimed to assess whether increased hepatic fatty-acid oxidation (FAO), at early ages, by gene transfer of Cpt1am (active mutant of carnitine palmitoyltransferase-1a), may be a strategy for reversing metabolic disturbances associated to maternal calorie-restriction during gestation in rats. Methods: AAV-Gfp (control) and AAV-Cpt1am vectors were administered by tail vein injection in 18-day-old control-pups and the offspring of 20% calorie-restricted rats during gestation (CR). After weaning, animals were fed with normal-fat diet. At the age of 4 months, they were moved to HF-diet and sacrificed at the age of 6 months to collect tissues. Locomotive activity, energy expenditure and blood pressure were measured. Results: Under HF-diet, CR-animals showed higher HOMA-IR, adipocyte diameter and hepatic triglyceride accumulation than controls; these alterations were reverted in Cpt1am-injected animals. In liver, this treatment ameliorated inflammatory state, decreased expression of lipogenesis-related genes and partially restored the decreased expression of leptin-receptor occurring in CR-animals. Treatment also reverted the decreased energy expenditure and the increased blood pressure of CR-animals. Conclusion: Increasing hepatic FAO through AAV-Cpt1am injection at juvenile ages prevents some metabolic disorders associated to gestational maternal calorie-restriction.
Subject
MESH
Malnutrition | Pregnancy, Animal | Humans | Metabolic Diseases | Carnitine O-Palmitoyltransferase | Fatty Acids | Fatty Liver | Pregnancy | Male | Genetic Therapy | Pregnancy Complications | Rats, Wistar | Female | Rats | Animals | Oxidation-Reduction | Prenatal Exposure Delayed Effects
DECS
Preñez | Animales | Ratas Wistar | Terapia Genética | Femenino | Masculino | Desnutrición | Enfermedades Metabólicas | Hígado Graso | Carnitina O-Palmitoiltransferasa | Ratas | Humanos | Ácidos Grasos | Complicaciones del Embarazo | Embarazo | Efectos Tardíos de la Exposición Prenatal | Oxidación-Reducción
Online version
DOI
Collections
Full text access