Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/16354
Título
CD44 Modulates Cell Migration and Invasion in Ewing Sarcoma Cells
Autor(es)
Fernández-Tabanera, Enrique ISCIII | Garcia-Garcia, Laura ISCIII | Rodriguez-Martin, Carlos ISCIII | Cervera Mayor, Saint Thomas ISCIII | Gonzalez-Gonzalez, Laura ISCIII | Robledo, Cristina ISCIII | Josa, Santiago ISCIII | Martinez Rodríguez, Selene ISCIII | Chapado, Luis ISCIII | Monzon-Fernandez, Sara ISCIII | Melero-Fernandez de Mera, Raquel M. ISCIII | Alonso, Javier ISCIII
Fecha de publicación
2023-07-21
Cita
Int J Mol Sci. 2023 Jul 21;24(14):11774.
Idioma
Inglés
Tipo de documento
research article
Resumen
The chimeric EWSR1::FLI1 transcription factor is the main oncogenic event in Ewing sarcoma. Recently, it has been proposed that EWSR1::FLI1 levels can fluctuate in Ewing sarcoma cells, giving rise to two cell populations. EWSR1::FLI1low cells present a migratory and invasive phenotype, while EWSR1::FLI1high cells are more proliferative. In this work, we described how the CD44 standard isoform (CD44s), a transmembrane protein involved in cell adhesion and migration, is overexpressed in the EWSR1::FLI1low phenotype. The functional characterization of CD44s (proliferation, clonogenicity, migration, and invasion ability) was performed in three doxycycline-inducible Ewing sarcoma cell models (A673, MHH-ES1, and CADO-ES1). As a result, CD44s expression reduced cell proliferation in all the cell lines tested without affecting clonogenicity. Additionally, CD44s increased cell migration in A673 and MHH-ES1, without effects in CADO-ES1. As hyaluronan is the main ligand of CD44s, its effect on migration ability was also assessed, showing that high molecular weight hyaluronic acid (HMW-HA) blocked cell migration while low molecular weight hyaluronic acid (LMW-HA) increased it. Invasion ability was correlated with CD44 expression in A673 and MHH-ES1 cell lines. CD44s, upregulated upon EWSR1::FLI1 knockdown, regulates cell migration and invasion in Ewing sarcoma cells.
Palabras clave
MESH
Sarcoma, Ewing | Humans | Hyaluronic Acid | Cell Line, Tumor | Oncogene Proteins, Fusion | Proto-Oncogene Protein c-fli-1 | Cell Movement | Gene Expression Regulation, Neoplastic | Hyaluronan Receptors
Versión en línea
DOI
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- Nombre:
- CD44ModulatesCellMigration_2023.pdf
- Tamaño:
- 4.185Mb
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- Descripción:
- Articulo
- Nombre:
- Supplementary_CD44ModulatesCel ...
- Tamaño:
- 446.6Kb
- Formato:
- Descripción:
- Supplementary material 1
- Nombre:
- TABLE_S1_CD44ModulatesCellMigr ...
- Tamaño:
- 1.062Mb
- Formato:
- Microsoft Excel 2007
- Descripción:
- Supplementary material 2