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dc.contributor.author | Fernández-Tabanera, Enrique | |
dc.contributor.author | Garcia-Garcia, Laura | |
dc.contributor.author | Rodriguez-Martin, Carlos | |
dc.contributor.author | Cervera Mayor, Saint Thomas | |
dc.contributor.author | Gonzalez-Gonzalez, Laura | |
dc.contributor.author | Robledo, Cristina | |
dc.contributor.author | Josa, Santiago | |
dc.contributor.author | Martinez Rodríguez, Selene | |
dc.contributor.author | Chapado, Luis | |
dc.contributor.author | Monzon-Fernandez, Sara | |
dc.contributor.author | Melero-Fernandez de Mera, Raquel M. | |
dc.contributor.author | Alonso, Javier | |
dc.date.accessioned | 2023-08-28T08:51:29Z | |
dc.date.available | 2023-08-28T08:51:29Z | |
dc.date.issued | 2023-07-21 | |
dc.identifier.citation | Int J Mol Sci. 2023 Jul 21;24(14):11774. | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/16354 | |
dc.description.abstract | The chimeric EWSR1::FLI1 transcription factor is the main oncogenic event in Ewing sarcoma. Recently, it has been proposed that EWSR1::FLI1 levels can fluctuate in Ewing sarcoma cells, giving rise to two cell populations. EWSR1::FLI1low cells present a migratory and invasive phenotype, while EWSR1::FLI1high cells are more proliferative. In this work, we described how the CD44 standard isoform (CD44s), a transmembrane protein involved in cell adhesion and migration, is overexpressed in the EWSR1::FLI1low phenotype. The functional characterization of CD44s (proliferation, clonogenicity, migration, and invasion ability) was performed in three doxycycline-inducible Ewing sarcoma cell models (A673, MHH-ES1, and CADO-ES1). As a result, CD44s expression reduced cell proliferation in all the cell lines tested without affecting clonogenicity. Additionally, CD44s increased cell migration in A673 and MHH-ES1, without effects in CADO-ES1. As hyaluronan is the main ligand of CD44s, its effect on migration ability was also assessed, showing that high molecular weight hyaluronic acid (HMW-HA) blocked cell migration while low molecular weight hyaluronic acid (LMW-HA) increased it. Invasion ability was correlated with CD44 expression in A673 and MHH-ES1 cell lines. CD44s, upregulated upon EWSR1::FLI1 knockdown, regulates cell migration and invasion in Ewing sarcoma cells. | es_ES |
dc.description.sponsorship | This project was funded by Instituto de Salud Carlos III, grant numbers PI20CIII/00020, DTS18CIII/00005, Asociación Pablo Ugarte, grant numbers TRPV205/18; Asociación Candela Riera, Asociación Todos Somos Iván & Fundación Sonrisa de Alex, grant numbers TVP333-19, TVP-1324/15; ASION, grant number TVP141/17. Enrique Fernández-Tabanera is supported by Asociación Candela Riera, Asociación Todos Somos Iván & Fundación Sonrisa de Alex, Saint T. Cervera is supported by Asociación Pablo Ugarte and Raquel M. Melero is supported by a CIBERER contract. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Ewing sarcoma | es_ES |
dc.subject | CD44 | es_ES |
dc.subject | EWSR1::FLI1 | es_ES |
dc.subject | Cell migration | es_ES |
dc.subject | Cell invasiveness | es_ES |
dc.subject.mesh | Sarcoma, Ewing | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Hyaluronic Acid | es_ES |
dc.subject.mesh | Cell Line, Tumor | es_ES |
dc.subject.mesh | Oncogene Proteins, Fusion | es_ES |
dc.subject.mesh | Proto-Oncogene Protein c-fli-1 | es_ES |
dc.subject.mesh | Cell Movement | es_ES |
dc.subject.mesh | Gene Expression Regulation, Neoplastic | es_ES |
dc.subject.mesh | Hyaluronan Receptors | es_ES |
dc.title | CD44 Modulates Cell Migration and Invasion in Ewing Sarcoma Cells | es_ES |
dc.type | research article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 37511533 | es_ES |
dc.format.volume | 24 | es_ES |
dc.format.number | 14 | es_ES |
dc.format.page | 11774 | es_ES |
dc.identifier.doi | 10.3390/ijms241411774 | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Asociación Pablo Ugarte contra el cáncer infantil | es_ES |
dc.contributor.funder | Candela Ribera. Asociación contra el sarcoma de Ewing | es_ES |
dc.contributor.funder | Asociación Todos somos Iván | es_ES |
dc.contributor.funder | Fundación la Sonrisa de Alex para la investigación y el tratamiento del sarcoma de Ewing | es_ES |
dc.contributor.funder | Centro de Investigación Biomédica en Red - CIBERER (Enfermedades Raras) | es_ES |
dc.contributor.funder | Asociación Infantil Oncológica de Madrid. La hucha de Tomás | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1422-0067 | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/ijms241411774 | es_ES |
dc.identifier.journal | International journal of molecular sciences | es_ES |
dc.repisalud.centro | ISCIII::Instituto de Investigación de Enfermedades Raras | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología::Unidades Comunes Científico-Técnicas (UCCT) | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFIS | info:fis/Instituto de Salud Carlos III/Programa Estatal de Generación de Conocimiento y Fortalecimiento del Sistema Español de I+D+I/Subprograma Estatal de Generación de Conocimiento/PI20-ISCIII Modalidad Proyectos de Investigacion en Salud Intramurales. (2020)/PI20CIII/00020 | es_ES |
dc.relation.projectFIS | info:fis/Instituto de Salud Carlos III/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/Subprograma Estatal de Generación de Conocimiento/DTS-ISCIII 2018 Modalidad Proyectos de Desarrollo Tecnológico en Salud Intramurales. (2018)/DTS18CIII/00005 | es_ES |