Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/10633
Exogenous, TAP-independent lysosomal presentation of a respiratory syncytial virus CTL epitope.
Immunol Cell Biol . 2012 Nov;90(10):978-82.
Respiratory syncytial virus causes lower respiratory tract infections in infancy and old age, affecting also immunocompromised patients. The viral fusion protein is an important vaccine candidate eliciting antibody and cell-mediated immune responses. CD8(+) cytotoxic T lymphocytes (CTLs) are known to have a role in both lung pathology and viral clearance. In BALB/c mice, the fusion protein epitope F249-258 is presented to CTLs by the murine major histocompatibility complex (MHC) class I molecule K(d). In cells infected with recombinant vaccinia viruses encoding the fusion protein, F249-258 is presented by MHC class I molecules through pathways that are independent of the transporters associated with antigen processing (TAP). We have now found that F249-258 can be generated from non-infectious virus from an exogenous source. Antigen processing follows a lysosomal pathway that appears to require autophagy. As a practical consequence, inactivated virus suffices for in vivo priming of virus-specific CTLs.
Antigen Presentation | Aged | Animals | Antigens, Ly | Antigens, Viral | Autophagy | Cell Line | Epitopes, T-Lymphocyte | Histocompatibility Antigens Class I | Humans | Immunocompromised Host | Infant | Lysosomes | Membrane Proteins | Mice | Mice, Inbred BALB C | Peptide Fragments | Recombinant Fusion Proteins | Respiratory Syncytial Virus Infections | Respiratory Syncytial Viruses | T-Lymphocytes, Cytotoxic | Viral Vaccines
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