Show simple item record

dc.contributor.authorRamos, Manuel 
dc.contributor.authorGarcia-Barreno, Blanca 
dc.contributor.authorLópez, Daniel 
dc.contributor.authorMelero, Jose Antonio 
dc.contributor.authorVal, Margarita del 
dc.contributor.authorJohnstone, Carolina
dc.identifier.citationImmunol Cell Biol . 2012 Nov;90(10):978-82.es_ES
dc.description.abstractRespiratory syncytial virus causes lower respiratory tract infections in infancy and old age, affecting also immunocompromised patients. The viral fusion protein is an important vaccine candidate eliciting antibody and cell-mediated immune responses. CD8(+) cytotoxic T lymphocytes (CTLs) are known to have a role in both lung pathology and viral clearance. In BALB/c mice, the fusion protein epitope F249-258 is presented to CTLs by the murine major histocompatibility complex (MHC) class I molecule K(d). In cells infected with recombinant vaccinia viruses encoding the fusion protein, F249-258 is presented by MHC class I molecules through pathways that are independent of the transporters associated with antigen processing (TAP). We have now found that F249-258 can be generated from non-infectious virus from an exogenous source. Antigen processing follows a lysosomal pathway that appears to require autophagy. As a practical consequence, inactivated virus suffices for in vivo priming of virus-specific CTLs.es_ES
dc.description.sponsorshipWe thank Dr. G. Hämmerling (German Cancer Research Centre, Heidelberg, Germany) for cell line T2/Kd. Recombinant human interleukin 2 was a gift of the NCI Preclinical Repository. Work in the laboratory is supported by grants from the Spanish Ministerio de Ciencia e Innovación and Redes Temáticas de Investigación Cooperativa del Instituto de Salud Carlos III. Technical assistance of C. Mir, S. Sánchez and Y. Laó is gratefully acknowledged, as well as peptide synthesis from Instituto de Salud Carlos III central facility.es_ES
dc.subject.meshAntigen Presentation es_ES
dc.subject.meshAged es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshAntigens, Ly es_ES
dc.subject.meshAntigens, Viral es_ES
dc.subject.meshAutophagy es_ES
dc.subject.meshCell Line es_ES
dc.subject.meshEpitopes, T-Lymphocyte es_ES
dc.subject.meshHistocompatibility Antigens Class I es_ES
dc.subject.meshHumans es_ES
dc.subject.meshImmunocompromised Host es_ES
dc.subject.meshInfant es_ES
dc.subject.meshLysosomes es_ES
dc.subject.meshMembrane Proteins es_ES
dc.subject.meshMice es_ES
dc.subject.meshMice, Inbred BALB C es_ES
dc.subject.meshPeptide Fragments es_ES
dc.subject.meshRecombinant Fusion Proteins es_ES
dc.subject.meshRespiratory Syncytial Virus Infections es_ES
dc.subject.meshRespiratory Syncytial Viruses es_ES
dc.subject.meshT-Lymphocytes, Cytotoxic es_ES
dc.subject.meshViral Vaccines es_ES
dc.titleExogenous, TAP-independent lysosomal presentation of a respiratory syncytial virus CTL epitope.es_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.identifier.journalImmunology and cell biologyes_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES

Files in this item

Acceso Abierto

This item appears in the following Collection(s)

Show simple item record

Atribución-NoComercial-CompartirIgual 4.0 Internacional
This item is licensed under a: Atribución-NoComercial-CompartirIgual 4.0 Internacional