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dc.contributor.authorMorales-Molina, Álvaro 
dc.contributor.authorGambera, Stefano 
dc.contributor.authorCejalvo, Teresa 
dc.contributor.authorMoreno, Rafael
dc.contributor.authorRodriguez-Milla, Miguel A 
dc.contributor.authorPerisé-Barrios, Ana Judith 
dc.contributor.authorGarcia-Castro, Javier
dc.identifier.citationCancer Immunol Immunother. 2018 Oct;67(10):1589-1602.es_ES
dc.descriptionFactor de impacto: 6,968 Q1
dc.description.abstractOncolytic virotherapy uses oncolytic viruses that selectively replicate in cancer cells. The use of cellular vehicles with migration ability to tumors has been considered to increase their delivery to target sites. Following this approach, the antitumor efficacy of the treatment Celyvir (mesenchymal stem cells infected with the oncolytic adenovirus ICOVIR-5) has been demonstrated in patients with neuroblastoma. However, the better efficacy of syngeneic or allogeneic mesenchymal stem cells as cell carriers and the specific role of the immune system in this therapy are still unknown. In this study we use our virotherapy Celyvir with syngeneic and allogeneic mouse mesenchymal stem cells to determine their antitumor efficacy in a C57BL/6 murine adenocarcinoma model. Adoptive transfer of splenocytes from treated mice to new tumor-bearing mice followed by a secondary adoptive transfer to a third group was performed. Similar reduction of tumor growth and systemic activation of the innate and adaptive immune system was observed in groups treated with syngeneic or allogeneic mesenchymal stem cells loaded with ICOVIR-5. Moreover, a different pattern of infiltration was observed by immunofluorescence in Celyvir-treated groups. While non-treated tumors presented higher density of infiltrating immune cells in the periphery of the tumor, both syngeneic and allogeneic Celyvir-treated groups presented higher infiltration of CD45+ cells in the core of the tumor. Therefore, these results suggest that syngeneic and allogeneic Celyvir induce systemic activation of the immune system, similar antitumor effect and a higher intratumoral infiltration of leukocytes.es_ES
dc.description.sponsorshipFunding for this research was provided by: Consejería de Educación, Juventud y Deporte, Comunidad de Madrid (P2010/BMD-2420) Asociación Pablo Ugarte Fondo de Investigaciones Sanitarias (PI17/00013, PI14CIII/00005) Fundación Oncohematología Infantil AFANIONes_ES
dc.publisherSpringer es_ES
dc.subject.meshOncolytic Virotherapy es_ES
dc.subject.meshAdenocarcinoma es_ES
dc.subject.meshAnimals es_ES
dc.subject.meshCarcinoma, Non-Small-Cell Lung es_ES
dc.subject.meshFemale es_ES
dc.subject.meshLung Neoplasms es_ES
dc.subject.meshLymphocytes, Tumor-Infiltrating es_ES
dc.subject.meshMesenchymal Stem Cells es_ES
dc.subject.meshMice es_ES
dc.subject.meshMice, Inbred C57BL es_ES
dc.subject.meshOncolytic Viruses es_ES
dc.subject.meshTumor Cells, Culturedes_ES
dc.titleAntitumor virotherapy using syngeneic or allogeneic mesenchymal stem cell carriers induces systemic immune response and intratumoral leukocyte infiltration in micees_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderComunidad de Madrid 
dc.identifier.journalCancer immunology, immunotherapy : CIIes_ES
dc.repisalud.centroISCIII::Instituto de Investigación de Enfermedades Rarases_ES

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Atribución-NoComercial-CompartirIgual 4.0 Internacional
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