Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/10621
Título
Antitumor virotherapy using syngeneic or allogeneic mesenchymal stem cell carriers induces systemic immune response and intratumoral leukocyte infiltration in mice
Autor(es)
Morales-Molina, Alvaro ISCIII | Gambera, Stefano ISCIII | Cejalvo, Teresa ISCIII | Moreno, Rafael | Rodriguez-Milla, Miguel A ISCIII | Perise-Barrios, Ana Judith ISCIII | Garcia-Castro, Javier ISCIII
Fecha de publicación
2018-10
Cita
Cancer Immunol Immunother. 2018 Oct;67(10):1589-1602.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Oncolytic virotherapy uses oncolytic viruses that selectively replicate in cancer cells. The use of cellular vehicles with migration ability to tumors has been considered to increase their delivery to target sites. Following this approach, the antitumor efficacy of the treatment Celyvir (mesenchymal stem cells infected with the oncolytic adenovirus ICOVIR-5) has been demonstrated in patients with neuroblastoma. However, the better efficacy of syngeneic or allogeneic mesenchymal stem cells as cell carriers and the specific role of the immune system in this therapy are still unknown. In this study we use our virotherapy Celyvir with syngeneic and allogeneic mouse mesenchymal stem cells to determine their antitumor efficacy in a C57BL/6 murine adenocarcinoma model. Adoptive transfer of splenocytes from treated mice to new tumor-bearing mice followed by a secondary adoptive transfer to a third group was performed. Similar reduction of tumor growth and systemic activation of the innate and adaptive immune system was observed in groups treated with syngeneic or allogeneic mesenchymal stem cells loaded with ICOVIR-5. Moreover, a different pattern of infiltration was observed by immunofluorescence in Celyvir-treated groups. While non-treated tumors presented higher density of infiltrating immune cells in the periphery of the tumor, both syngeneic and allogeneic Celyvir-treated groups presented higher infiltration of CD45+ cells in the core of the tumor. Therefore, these results suggest that syngeneic and allogeneic Celyvir induce systemic activation of the immune system, similar antitumor effect and a higher intratumoral infiltration of leukocytes.
MESH
Oncolytic Virotherapy | Adenocarcinoma | Animals | Carcinoma, Non-Small-Cell Lung | Female | Lung Neoplasms | Lymphocytes, Tumor-Infiltrating | Mesenchymal Stem Cells | Mice | Mice, Inbred C57BL | Oncolytic Viruses | Tumor Cells, Cultured
Descripción
Factor de impacto: 6,968 Q1
Versión en línea
DOI
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