Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/9262
Título
Clonal dynamics in osteosarcoma defined by RGB marking
Autor(es)
Gambera, Stefano ISCIII | Abarrategi, Ander ISCIII | Garcia-Castro, Javier ISCIII | Morales-Molina, Alvaro ISCIII | Roma, Josep | Alfranca, Arantzazu ISCIII | Gonzalez-Camacho, Fernando ISCIII
Fecha de publicación
2018
Cita
Nat Commun. 2018 Sep 28;9(1):3994.
Idioma
Inglés
Tipo de documento
journal article
Resumen
Osteosarcoma is a type of bone tumour characterized by considerable levels of phenotypic heterogeneity, aneuploidy, and a high mutational rate. The life expectancy of osteosarcoma patients has not changed during the last three decades and thus much remains to be learned about the disease biology. Here, we employ a RGB-based single-cell tracking system to study the clonal dynamics occurring in a de novo-induced murine osteosarcoma model. We show that osteosarcoma cells present initial polyclonal dynamics, followed by clonal dominance associated with adaptation to the microenvironment. Interestingly, the dominant clones are composed of subclones with a similar tumour generation potential when they are re-implanted in mice. Moreover, individual spontaneous metastases are clonal or oligoclonal, but they have a different cellular origin than the dominant clones present in primary tumours. In summary, we present evidence that osteosarcomagenesis can follow a neutral evolution model, in which different cancer clones coexist and propagate simultaneously.
MESH
Animals | Bone Neoplasms | Clone Cells | Luminescent Proteins | Mesenchymal Stem Cells | Mice, Inbred NOD | Mice, Knockout | Mice, SCID | Mice, Transgenic | Microscopy, Confocal | Osteosarcoma | Single-Cell Analysis
Descripción
Factor de impacto: 14,919
Versión en línea
DOI
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