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dc.contributor.authorGambera, Stefano 
dc.contributor.authorAbarrategi, Ander 
dc.contributor.authorGarcia-Castro, Javier 
dc.contributor.authorMorales-Molina, Álvaro 
dc.contributor.authorRoma, Josep
dc.contributor.authorAlfranca, Arantzazu 
dc.contributor.authorGonzález-Camacho, Fernando
dc.identifier.citationNat Commun. 2018 Sep 28;9(1):3994.es_ES
dc.description.abstractOsteosarcoma is a type of bone tumour characterized by considerable levels of phenotypic heterogeneity, aneuploidy, and a high mutational rate. The life expectancy of osteosarcoma patients has not changed during the last three decades and thus much remains to be learned about the disease biology. Here, we employ a RGB-based single-cell tracking system to study the clonal dynamics occurring in a de novo-induced murine osteosarcoma model. We show that osteosarcoma cells present initial polyclonal dynamics, followed by clonal dominance associated with adaptation to the microenvironment. Interestingly, the dominant clones are composed of subclones with a similar tumour generation potential when they are re-implanted in mice. Moreover, individual spontaneous metastases are clonal or oligoclonal, but they have a different cellular origin than the dominant clones present in primary tumours. In summary, we present evidence that osteosarcomagenesis can follow a neutral evolution model, in which different cancer clones coexist and propagate simultaneously.es_ES
dc.description.sponsorshipWe thank ISCIII and CNIO flow cytometry and cell sorting units for their participation in our studies. We are thankful to the CCEH-Fred Hutchinson Cancer Research Center for LAM-PCR service. We acknowledge Raquel Pérez Tavarez, María Blázquez Mesa, Alicia Giménez Sánchez, Elena Calvo Cazalilla, and Monserrat Arroyo Correas for useful help on the pathology studies; and Teresa Cejalvo, Isabel Cubillo Moreno, and Miguel Angel Rodríguez-Milla for their contributions in experimental setup. We thank the visual artist Isabella Lacquaniti for her help with drawings and schematics. We are also thankful to the Fondo de Investigaciones Sanitarias (FIS: PI11/00377 and PI14CIII/00005 to J.G.-C., FIS: CP11/00206 to A.A., and RTICC: RD12/0036/0027 to J.G.-C.), the Madrid Regional Government (CellCAM; P2010/BMD-2420 to J.G.-C.), the Asociación Pablo Ugarte, and the Asociación Afanion for grants support.es_ES
dc.publisherNature Researches_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subject.meshAnimals es_ES
dc.subject.meshBone Neoplasms es_ES
dc.subject.meshClone Cells es_ES
dc.subject.meshLuminescent Proteins es_ES
dc.subject.meshMesenchymal Stem Cells es_ES
dc.subject.meshMice, Inbred NOD es_ES
dc.subject.meshMice, Knockout es_ES
dc.subject.meshMice, SCID es_ES
dc.subject.meshMice, Transgenic es_ES
dc.subject.meshMicroscopy, Confocal es_ES
dc.subject.meshOsteosarcoma es_ES
dc.subject.meshSingle-Cell Analysis es_ES
dc.titleClonal dynamics in osteosarcoma defined by RGB markinges_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderFondo de Investigaciones Sanitariases_ES
dc.contributor.funderGobierno de la Comunidad Autónoma de Madrides_ES
dc.identifier.journalNature communicationses_ES
dc.repisalud.centroISCIII::Instituto de Investigación de Enfermedades Rarases_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.relation.projectIDInfo:eu-repo/grantAgreement/FIS: CP11/00206es_ES
dc.relation.projectIDInfo:eu-repo/grantAgreement/RTICC: RD12/0036/0027es_ES
dc.relation.projectIDInfo:eu-repo/grantAgreement/CellCAM; P2010/BMD-2420es_ES

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Atribución 4.0 Internacional
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