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dc.contributor.author | Suárez, Henar | |
dc.contributor.author | López-Martín, Soraya | |
dc.contributor.author | Toribio, Victor | |
dc.contributor.author | Zamai, Moreno | |
dc.contributor.author | Hernandez-Riquer, Maria Victoria | |
dc.contributor.author | Genis, Laura | |
dc.contributor.author | Arroyo, Alicia G | |
dc.contributor.author | Yanez-Mo, Maria | |
dc.date.accessioned | 2020-03-09T13:18:56Z | |
dc.date.available | 2020-03-09T13:18:56Z | |
dc.date.issued | 2020-02 | |
dc.identifier.citation | Cells. 2020; 9(2):348 | es_ES |
dc.identifier.issn | 2073-4409 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/9244 | |
dc.description.abstract | Membrane-bound proteases play a key role in biology by degrading matrix proteins or shedding adhesion receptors. MT1-MMP metalloproteinase is critical during cancer invasion, angiogenesis, and development. MT1-MMP activity is strictly regulated by internalization, recycling, autoprocessing but also through its incorporation into tetraspanin-enriched microdomains (TEMs), into invadopodia, or by its secretion on extracellular vesicles (EVs). We identified a juxtamembrane positively charged cluster responsible for the interaction of MT1-MMP with ERM (ezrin/radixin/moesin) cytoskeletal connectors in breast carcinoma cells. Linkage to ERMs regulates MT1-MMP subcellular distribution and internalization, but not its incorporation into extracellular vesicles. MT1-MMP association to ERMs and insertion into TEMs are independent phenomena, so that mutation of the ERM-binding motif in the cytoplasmic region of MT1-MMP does not preclude its association with the tetraspanin CD151, but impairs the accumulation and coalescence of CD151/MT1-MMP complexes at actin-rich structures. Conversely, gene deletion of CD151 does not impact on MT1-MMP colocalization with ERM molecules. At the plasma membrane MT1-MMP autoprocessing is severely dependent on ERM association and seems to be the dominant regulator of the enzyme collagenolytic activity. This newly characterized MT1-MMP/ERM association can thus be of relevance for tumor cell invasion. | es_ES |
dc.description.sponsorship | This work has been supported by grants BFU2014-55478-R, REDIEX. SAF2015-71231-REDT and BIO2017-86500-R from Ministerio Español de Economía y Competitividad (MINECO) and by a grant from Fundación Ramón Areces “Ayudas a la Investigación en Ciencias de la Vida y de la Materia, 2014” to M.Y.-M. H.S. was supported by a FPI-UAM fellowship. The CNIC is supported by the Ministry of Ciencia, Innovacion y Universidades and the Pro CNIC Foundation, is a Severo Ochoa Center of Excellence (SEV-2015-0505), also supported by European Regional Development Fund (FEDER) “Una manera de hacer Europa”. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | ERM | es_ES |
dc.subject | MT1-MMP | es_ES |
dc.subject | Extracellular vesicles | es_ES |
dc.subject | Tetraspanin enriched-microdomains | es_ES |
dc.title | Regulation of MT1-MMP Activity through Its Association with ERMs | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 32028690 | es_ES |
dc.format.volume | 9 | es_ES |
dc.format.number | 2 | es_ES |
dc.format.page | 348 | es_ES |
dc.identifier.doi | 10.3390/cells9020348 | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Fundación Ramón Areces | |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Fundación ProCNIC | |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (España) | |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2073-4409 | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/cells9020348 | es_ES |
dc.identifier.journal | Cells | es_ES |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Metaloproteinasas de Matriz en Angiogénesis e Inflamación | es_ES |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Microscopía | es_ES |
dc.repisalud.institucion | CNIC | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SEV-2015-0505 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/BFU2014-55478-R | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2015-71231-REDT | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/BIO2017-86500-R | es_ES |
dc.rights.accessRights | open access | es_ES |