Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/9244
Título
Regulation of MT1-MMP Activity through Its Association with ERMs
Autor(es)
Suárez, Henar | López-Martín, Soraya | Toribio, Victor | Zamai, Moreno CNIC | Hernandez-Riquer, Maria Victoria CNIC | Genis, Laura CNIC | Arroyo, Alicia G CNIC | Yanez-Mo, Maria
Fecha de publicación
2020-02
Cita
Cells. 2020; 9(2):348
Idioma
Inglés
Tipo de documento
journal article
Resumen
Membrane-bound proteases play a key role in biology by degrading matrix proteins or shedding adhesion receptors. MT1-MMP metalloproteinase is critical during cancer invasion, angiogenesis, and development. MT1-MMP activity is strictly regulated by internalization, recycling, autoprocessing but also through its incorporation into tetraspanin-enriched microdomains (TEMs), into invadopodia, or by its secretion on extracellular vesicles (EVs). We identified a juxtamembrane positively charged cluster responsible for the interaction of MT1-MMP with ERM (ezrin/radixin/moesin) cytoskeletal connectors in breast carcinoma cells. Linkage to ERMs regulates MT1-MMP subcellular distribution and internalization, but not its incorporation into extracellular vesicles. MT1-MMP association to ERMs and insertion into TEMs are independent phenomena, so that mutation of the ERM-binding motif in the cytoplasmic region of MT1-MMP does not preclude its association with the tetraspanin CD151, but impairs the accumulation and coalescence of CD151/MT1-MMP complexes at actin-rich structures. Conversely, gene deletion of CD151 does not impact on MT1-MMP colocalization with ERM molecules. At the plasma membrane MT1-MMP autoprocessing is severely dependent on ERM association and seems to be the dominant regulator of the enzyme collagenolytic activity. This newly characterized MT1-MMP/ERM association can thus be of relevance for tumor cell invasion.
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