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dc.contributor.authorCalero, Olga 
dc.contributor.authorGarcia-Albert, Luis 
dc.contributor.authorRodríguez-Martín, Andrés
dc.contributor.authorVeiga, Sergio
dc.contributor.authorCalero, Miguel
dc.identifier.citationSci Rep. 2018 Apr 13;8(1):5969.es_ES
dc.description.abstractApolipoprotein E (apoE) is a 34 kDa glycoprotein involved in lipid metabolism. The human APOE gene encodes for three different apoE protein isoforms: E2, E3 and E4. The interest in apoE isoforms is high for epidemiological research, patient stratification and identification of those at increased risk for clinical trials and prevention. The isoform apoE4 is associated with increased risk for coronary heart and Alzheimer's diseases. This paper describes a method for specifically detecting the apoE4 isoform from biological fluids by taking advantage of the capacity of apoE to bind "specifically" to polystyrene surfaces as capture and a specific anti-apoE4 monoclonal antibody as reporter. Our results indicate that the apoE-polystyrene binding interaction is highly stable, resistant to detergents and acid and basic washes. The methodology here described is accurate, easily implementable, fast and cost-effective. Although at present, our technique is unable to discriminate homozygous APOE ε4/ε4 from APOE ε3/ε4 and ε2/ε4 heterozygous, it opens new avenues for the development of inexpensive, yet effective, tests for the detection of apoE4 for patients' stratification. Preliminary results indicated that this methodology is also adaptable into turbidimetric platforms, which make it a good candidate for clinical implementation through its translation to the clinical analysis routine.es_ES
dc.description.sponsorshipThe authors thank all the patients, hospitals, and institutions that made this study possible. We thank Pablo Cabello and Carlo Zanotti for their decisive role throughout the course of this project, and Elisenda Rodón, Almudena Pérez and Sergi Gassó from Pragmatic Diagnostics (Barcelona, Spain) for their work on the adaptation of the method to immunoturbidimetry. We also thank Montserrat Puntes (MD.) and Maria Teresa Garrido (MSc.) from the Centre for Drug Research (CIM) of the Hospital de la Santa Creu i Sant Pau for their collaboration on the project entitled “Study for the development of a non-genetic test in blood for the identification of carriers of the ε4 alelle of the APOE gene (APOE ε4)” (Code: BCR-2017-01).es_ES
dc.publisherNature Publishing Group es_ES
dc.subject.meshAlzheimer Disease es_ES
dc.subject.meshAntibodies, Monoclonal es_ES
dc.subject.meshApolipoproteins E es_ES
dc.subject.meshCohort Studies es_ES
dc.subject.meshCoronary Disease es_ES
dc.subject.meshCost-Benefit Analysis es_ES
dc.subject.meshGenotype es_ES
dc.subject.meshHeterozygote es_ES
dc.subject.meshHomozygote es_ES
dc.subject.meshHumans es_ES
dc.subject.meshPolystyrenes es_ES
dc.subject.meshProtein Isoforms es_ES
dc.titleA fast and cost-effective method for apolipoprotein E isotyping as an alternative to APOE genotyping for patient screening and stratificationes_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.journalScientific reportses_ES
dc.repisalud.centroISCIII::Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC)es_ES
dc.rights.accessRightsopen accesses_ES

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