dc.contributor.author | Calero, Olga | |
dc.contributor.author | Garcia-Albert, Luis | |
dc.contributor.author | Rodríguez-Martín, Andrés | |
dc.contributor.author | Veiga, Sergio | |
dc.contributor.author | Calero, Miguel | |
dc.date.accessioned | 2020-02-26T15:26:48Z | |
dc.date.available | 2020-02-26T15:26:48Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Sci Rep. 2018 Apr 13;8(1):5969. | es_ES |
dc.identifier.issn | 2045-2322 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/9148 | |
dc.description.abstract | Apolipoprotein E (apoE) is a 34 kDa glycoprotein involved in lipid metabolism. The human APOE gene encodes for three different apoE protein isoforms: E2, E3 and E4. The interest in apoE isoforms is high for epidemiological research, patient stratification and identification of those at increased risk for clinical trials and prevention. The isoform apoE4 is associated with increased risk for coronary heart and Alzheimer's diseases. This paper describes a method for specifically detecting the apoE4 isoform from biological fluids by taking advantage of the capacity of apoE to bind "specifically" to polystyrene surfaces as capture and a specific anti-apoE4 monoclonal antibody as reporter. Our results indicate that the apoE-polystyrene binding interaction is highly stable, resistant to detergents and acid and basic washes. The methodology here described is accurate, easily implementable, fast and cost-effective. Although at present, our technique is unable to discriminate homozygous APOE ε4/ε4 from APOE ε3/ε4 and ε2/ε4 heterozygous, it opens new avenues for the development of inexpensive, yet effective, tests for the detection of apoE4 for patients' stratification. Preliminary results indicated that this methodology is also adaptable into turbidimetric platforms, which make it a good candidate for clinical implementation through its translation to the clinical analysis routine. | es_ES |
dc.description.sponsorship | The authors thank all the patients, hospitals, and institutions that made this study possible. We thank Pablo Cabello and Carlo Zanotti for their decisive role throughout the course of this project, and Elisenda Rodón, Almudena Pérez and Sergi Gassó from Pragmatic Diagnostics (Barcelona, Spain) for their work on the adaptation of the method to immunoturbidimetry. We also thank Montserrat Puntes (MD.) and Maria Teresa Garrido (MSc.) from the Centre for Drug Research (CIM) of the Hospital de la Santa Creu i Sant Pau for their collaboration on the project entitled “Study for the development of a non-genetic test in blood for the identification of carriers of the ε4 alelle of the APOE gene (APOE ε4)” (Code: BCR-2017-01). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | Alzheimer Disease | es_ES |
dc.subject.mesh | Antibodies, Monoclonal | es_ES |
dc.subject.mesh | Apolipoproteins E | es_ES |
dc.subject.mesh | Cohort Studies | es_ES |
dc.subject.mesh | Coronary Disease | es_ES |
dc.subject.mesh | Cost-Benefit Analysis | es_ES |
dc.subject.mesh | Genotype | es_ES |
dc.subject.mesh | Heterozygote | es_ES |
dc.subject.mesh | Homozygote | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Polystyrenes | es_ES |
dc.subject.mesh | Protein Isoforms | es_ES |
dc.title | A fast and cost-effective method for apolipoprotein E isotyping as an alternative to APOE genotyping for patient screening and stratification | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 29654261 | es_ES |
dc.format.volume | 8 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 5969 | es_ES |
dc.identifier.doi | 10.1038/s41598-018-24320-3 | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2045-2322 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1038/s41598-018-24320-3 | es_ES |
dc.identifier.journal | Scientific reports | es_ES |
dc.repisalud.centro | ISCIII::Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC) | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |