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dc.contributor.author | Fernández-Calvet, Ariadna | |
dc.contributor.author | Rodríguez-Arce, Irene | |
dc.contributor.author | Almagro, Goizeder | |
dc.contributor.author | Moleres, Javier | |
dc.contributor.author | Euba, Begoña | |
dc.contributor.author | Caballero, Lucía | |
dc.contributor.author | Martí, Sara | |
dc.contributor.author | Ramos-Vivas, José | |
dc.contributor.author | Bartholomew, Toby Leigh | |
dc.contributor.author | Morales, Xabier | |
dc.contributor.author | Ortíz-de-Solórzano, Carlos | |
dc.contributor.author | Yuste, Jose Enrique | |
dc.contributor.author | Bengoechea, José Antonio | |
dc.contributor.author | Conde-Álvarez, Raquel | |
dc.contributor.author | Garmendia, Junkal | |
dc.date.accessioned | 2020-02-25T12:12:48Z | |
dc.date.available | 2020-02-25T12:12:48Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Sci Rep. 2018 May 2;8(1):6872. | es_ES |
dc.identifier.issn | 2045-2322 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/9147 | |
dc.description.abstract | Airway infection by nontypeable Haemophilus influenzae (NTHi) associates to chronic obstructive pulmonary disease (COPD) exacerbation and asthma neutrophilic airway inflammation. Lipids are key inflammatory mediators in these disease conditions and consequently, NTHi may encounter free fatty acids during airway persistence. However, molecular information on the interplay NTHi-free fatty acids is limited, and we lack evidence on the importance of such interaction to infection. Maintenance of the outer membrane lipid asymmetry may play an essential role in NTHi barrier function and interaction with hydrophobic molecules. VacJ/MlaA-MlaBCDEF prevents phospholipid accumulation at the bacterial surface, being the only system involved in maintaining membrane asymmetry identified in NTHi. We assessed the relationship among the NTHi VacJ/MlaA outer membrane lipoprotein, bacterial and exogenous fatty acids, and respiratory infection. The vacJ/mlaA gene inactivation increased NTHi fatty acid and phospholipid global content and fatty acyl specific species, which in turn increased bacterial susceptibility to hydrophobic antimicrobials, decreased NTHi epithelial infection, and increased clearance during pulmonary infection in mice with both normal lung function and emphysema, maybe related to their shared lung fatty acid profiles. Altogether, we provide evidence for VacJ/MlaA as a key bacterial factor modulating NTHi survival at the human airway upon exposure to hydrophobic molecules. | es_ES |
dc.description.sponsorship | We thank Dr. Víctor de Lorenzo (CNB-CSIC, Spain) for his support, Dr. Ignacio Moriyón (Universidad de Navarra, Spain) for helpful discussions, Dr. Javier Pozueta for allowing access to a GC-MS equipment, Dr. Auxiliadora Prieto (CIB-CSIC, Spain) for allowing access to a Multiskan device. A.F.C was funded by a contract from Ministerio Economía y Competitividad-MINECO, reference 20132RC947, Spain; I.R.A. is funded by a PhD studentship from Universidad Pública de Navarra, Spain; J.M. was funded by PhD studentship BES-2013-062644 from MINECO; S.M. is funded by a postdoctoral contract from CIBERES; L.C. was funded by a contract from MINECO, reference CS_NAV_IDAB_005, Spain; T.L.B. is the recipient of a PhD fellowship funded by the Department for Employment and Learning (Northern Ireland, UK). This work has been funded by grants from MINECO SAF2012-31166 and SAF2015-66520-R, from Health Department, Regional Govern from Navarra, Spain, reference 03/2016, and from SEPAR 31/2015 to J.G.; and by grant from MINECO DPI2015-64221 to COdS. CIBER is an initiative from Instituto de Salud Carlos III (ISCIII), Madrid, Spain. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature Publishing Group | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | Animals | es_ES |
dc.subject.mesh | Bacterial Outer Membrane Proteins | es_ES |
dc.subject.mesh | Cell Line | es_ES |
dc.subject.mesh | Cell Line, Tumor | es_ES |
dc.subject.mesh | Cell Membrane | es_ES |
dc.subject.mesh | Fatty Acids | es_ES |
dc.subject.mesh | Female | es_ES |
dc.subject.mesh | Haemophilus Infections | es_ES |
dc.subject.mesh | Haemophilus influenzae | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Lipoproteins | es_ES |
dc.subject.mesh | Mice | es_ES |
dc.subject.mesh | Phospholipid Transfer Proteins | es_ES |
dc.subject.mesh | Respiratory Mucosa | es_ES |
dc.title | Modulation of Haemophilus influenzae interaction with hydrophobic molecules by the VacJ/MlaA lipoprotein impacts strongly on its interplay with the airways | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 29720703 | es_ES |
dc.format.volume | 8 | es_ES |
dc.format.number | 1 | es_ES |
dc.format.page | 6872 | es_ES |
dc.identifier.doi | 10.1038/s41598-018-25232-y | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Comunidad Foral de Navarra (España) | |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Centro de Investigación Biomedica en Red - CIBER | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 2045-2322 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1038/s41598-018-25232-y | es_ES |
dc.identifier.journal | Scientific reports | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/20132RC947 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/BES-2013-062644 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/SAF2012-31166 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2015-66520-R | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/03/2016 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/31/2015 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/DPI2015-64221 | es_ES |
dc.rights.accessRights | open access | es_ES |