Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/9001
Título
Y155H amino acid substitution in influenza A(H1N1)pdm09 viruses does not confer a phenotype of reduced susceptibility to neuraminidase inhibitors
Autor(es)
Perez-Sautu, Unai ISCIII | Pozo Sanchez, Francisco ISCIII | Cuesta de la Plaza, Isabel ISCIII | Monzon-Fernandez, Sara ISCIII | Calderon-Reñon, Ana Maria ISCIII | Gonzalez, M | Molinero, Mar ISCIII | Lopez-Miragaya, I | Rey, Sonia | Cañizares, A | Rodriguez, G | Gonzalez-Velasco, C | Lackenby, A | Casas Flecha, Inmaculada ISCIII
Fecha de publicación
2014-07-10
Cita
Euro Surveill. 2014 Jul 10;19(27):14-20.
Idioma
Inglés
Tipo de documento
journal article
Resumen
The Y155H amino acid substitution in the neuraminidase gene (NA) has previously been associated with highly reduced inhibition by neuraminidase inhibitors in the seasonal H1N1 influenza A virus which circulated in humans before the 2009 pandemic. During the 2012/13 epidemic season in Spain, two A(H1N1) pdm09 viruses bearing the specific Y155H substitution in the NA were detected and isolated from two patients diagnosed with severe respiratory syndrome and pneumonia requiring admission to the intensive care unit. Contrary to what was observed in the seasonal A(H1N1) viruses, neither of the Y155H A(H1N1) pdm09 viruses described here showed a phenotype of reduced inhibition by NAIs as determined by the neuraminidase enzyme inhibition assay (MUNANA). High-throughput sequencing of the NA of both Y155H viruses showed that they were composed to >99% of H155 variants. We believe that this report can contribute to a better understanding of the biological significance of amino acid substitutions in the neuraminidase protein with regard to susceptibility of influenza viruses to neuraminidase inhibitors. This is of critical importance for optimal management of influenza disease patients.
MESH
Amino Acid Substitution | Antiviral Agents | Drug Resistance, Viral | Enzyme Inhibitors | Female | Humans | Immunoenzyme Techniques | Influenza A Virus, H1N1 Subtype | Influenza, Human | Male | Microbial Sensitivity Tests | Neuraminidase | Oseltamivir | Pandemics | Phenotype | RNA, Viral | Seasons | Sequence Analysis, DNA | Spain | Viral Proteins | Zanamivir
Versión en línea
DOI
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