2024-03-28T20:11:01Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/90012022-09-29T10:47:22Zcom_20.500.12105_2060com_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_2061
00925njm 22002777a 4500
dc
Perez-Sautu, Unai
author
Pozo Sanchez, Francisco
author
Cuesta de la Plaza, Isabel
author
Monzon-Fernandez, Sara
author
Calderon-Reñon, Ana Maria
author
Gonzalez, M
author
Molinero, Mar
author
Lopez-Miragaya, I
author
Rey, Sonia
author
Cañizares, A
author
Rodriguez, G
author
Gonzalez-Velasco, C
author
Lackenby, A
author
Casas Flecha, Inmaculada
author
2014-07-10
The Y155H amino acid substitution in the neuraminidase gene (NA) has previously been associated with highly reduced inhibition by neuraminidase inhibitors in the seasonal H1N1 influenza A virus which circulated in humans before the 2009 pandemic. During the 2012/13 epidemic season in Spain, two A(H1N1) pdm09 viruses bearing the specific Y155H substitution in the NA were detected and isolated from two patients diagnosed with severe respiratory syndrome and pneumonia requiring admission to the intensive care unit. Contrary to what was observed in the seasonal A(H1N1) viruses, neither of the Y155H A(H1N1) pdm09 viruses described here showed a phenotype of reduced inhibition by NAIs as determined by the neuraminidase enzyme inhibition assay (MUNANA). High-throughput sequencing of the NA of both Y155H viruses showed that they were composed to >99% of H155 variants. We believe that this report can contribute to a better understanding of the biological significance of amino acid substitutions in the neuraminidase protein with regard to susceptibility of influenza viruses to neuraminidase inhibitors. This is of critical importance for optimal management of influenza disease patients.
Euro Surveill. 2014 Jul 10;19(27):14-20.
http://hdl.handle.net/20.500.12105/9001
25033052
10.2807/1560-7917.ES2014.19.27.20849
1560-7917
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
Y155H amino acid substitution in influenza A(H1N1)pdm09 viruses does not confer a phenotype of reduced susceptibility to neuraminidase inhibitors