Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/8984
Título
Genomic non-coding regions reveal hidden patterns of mumps virus circulation in Spain, 2005 to 2015
Autor(es)
Gavilán, Ana M ISCIII | Fernandez-Garcia, Aurora ISCIII | Rueda,, Angel | Castellanos-Nadal, Ana Maria ISCIII | Masa-Calles, Josefa ISCIII | Lopez-Perea, Noemi ISCIII | Torres-de Mier, Maria de Viarce ISCIII | De Ory, Fernando de ISCIII | Echevarria, Juan Emilio ISCIII
Fecha de publicación
2018
Cita
Euro Surveill. 2018 Apr;23(15):17-00349.
Idioma
Inglés
Tipo de documento
research article
Resumen
BackgroundSince mumps vaccination was introduced in 1981 in Spain, the incidence of the disease has dropped significantly. However, cyclic epidemic waves and outbreaks still occur, despite high vaccination coverage. The World Health Organization (WHO) recommends genotyping to trace the pattern of mumps virus (MuV) circulation. Genotype H was predominant in Spain, but was replaced in 2005 by genotype G which has subsequently remained dominant. Of the small hydrophobic protein gene sequences, 78% are identical and belong to the MuVi/ Sheffield.GBR.1.05/[G]-variant. Aim: Our study aimed to investigate whether the circulation of MuV strains in Spain was continuous after the emergence of genotype G in 2005. Method: We obtained 46 samples from Spanish patients infected with MuVi/Sheffield.GBR.1.05/[G] during two epidemic waves and analysed them using new molecular markers based on genomic non-coding regions (NCRs) that discriminate subvariants of this virus strain. Results: Phylogenetic analyses of the nucleoprotein-phosphoprotein and matrix protein-fusion protein NCR indicated strain replacement after a drop in incidence in 2009, which had not been detectable by SH sequencing. Clustering of sequences from patients epidemiologically linked in the same outbreak suggests a potential use for these NCRs in outbreak characterisation. Conclusion: We suggest to consider their use in conjunction with the SH gene in the future WHO recommendations for MuV epidemiological surveillance.
Palabras clave
MESH
Cluster Analysis | Genomics | Genotype | Humans | Molecular Epidemiology | Molecular Sequence Data | Mumps | Mumps virus | Phylogeny | RNA, Untranslated | RNA, Viral | Sequence Analysis, DNA | Spain | Disease Outbreaks
Versión en línea
DOI
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