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dc.contributor.authorGavilán, Ana M 
dc.contributor.authorFernández-García, Aurora 
dc.contributor.authorRueda, Angel 
dc.contributor.authorCastellanos-Nadal, Ana Maria 
dc.contributor.authorMasa-Calles, Josefa 
dc.contributor.authorLópez-Perea, Noemí 
dc.contributor.authorTorres-de Mier, Maria Viarce 
dc.contributor.authorOry, Fernando de 
dc.contributor.authorEchevarria, Juan Emilio
dc.identifier.citationEuro Surveill. 2018 Apr;23(15).es_ES
dc.description.abstractBackgroundSince mumps vaccination was introduced in 1981 in Spain, the incidence of the disease has dropped significantly. However, cyclic epidemic waves and outbreaks still occur, despite high vaccination coverage. The World Health Organization (WHO) recommends genotyping to trace the pattern of mumps virus (MuV) circulation. Genotype H was predominant in Spain, but was replaced in 2005 by genotype G which has subsequently remained dominant. Of the small hydrophobic protein gene sequences, 78% are identical and belong to the MuVi/ Sheffield.GBR.1.05/[G]-variant. Aim: Our study aimed to investigate whether the circulation of MuV strains in Spain was continuous after the emergence of genotype G in 2005. Method: We obtained 46 samples from Spanish patients infected with MuVi/Sheffield.GBR.1.05/[G] during two epidemic waves and analysed them using new molecular markers based on genomic non-coding regions (NCRs) that discriminate subvariants of this virus strain. Results: Phylogenetic analyses of the nucleoprotein-phosphoprotein and matrix protein-fusion protein NCR indicated strain replacement after a drop in incidence in 2009, which had not been detectable by SH sequencing. Clustering of sequences from patients epidemiologically linked in the same outbreak suggests a potential use for these NCRs in outbreak characterisation. Conclusion: We suggest to consider their use in conjunction with the SH gene in the future WHO recommendations for MuV epidemiological surveillance.es_ES
dc.description.sponsorshipWe would like to thank the Genomic Unit of the CNM-ISCIII for their technical assistance with sequencing. This work was supported by the “Fondo de Investigación Sanitaria” (FIS PI12/02006) and the “Instituto de Salud Carlos III” (PI15CIII/00023). Aurora Fernández García was funded by CIBER de Epidemiología y Salud Pública (CIBERESP), ISCIII.es_ES
dc.publisherEuropean Centre for Disease Prevention and Controles_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subjectgenotype Ges_ES
dc.subjectmolecular epidemiologyes_ES
dc.subjectmolecular methodses_ES
dc.subjectmumps viruses_ES
dc.subjectnon-coding regionses_ES
dc.subject.meshCluster Analysis es_ES
dc.subject.meshGenomics es_ES
dc.subject.meshGenotype es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMolecular Epidemiology es_ES
dc.subject.meshMolecular Sequence Data es_ES
dc.subject.meshMumps es_ES
dc.subject.meshMumps virus es_ES
dc.subject.meshPhylogeny es_ES
dc.subject.meshRNA, Untranslated es_ES
dc.subject.meshRNA, Viral es_ES
dc.subject.meshSequence Analysis, DNA es_ES
dc.subject.meshSpain es_ES
dc.subject.meshDisease Outbreaks es_ES
dc.titleGenomic non-coding regions reveal hidden patterns of mumps virus circulation in Spain, 2005 to 2015es_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.contributor.funderFondo de Investigaciones Sanitariases_ES
dc.contributor.funderInstituto de Salud Carlos III - ISCIIIes_ES
dc.identifier.journalEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletines_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.centroISCIII::Centro Nacional de Epidemologíaes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/FIS PI12/02006es_ES

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Atribución 4.0 Internacional
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