Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/8278
Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region
Casas Flecha, Inmaculada ISCIII | Avellon, Ana ISCIII | Mosquera Gutierrez, Maria del Mar ISCIII | Jabado, O | Echevarria, Juan Emilio ISCIII | Campos, R H | Rewers, M | Perez-Breña, Pilar ISCIII | Lipkin, W I | Palacios, G
J Clin Microbiol. 2005 Dec;43(12):6176-82.
Here we present a system for adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserved hexon gene fragment. The system was validated using 157 sequences (86 previously typed and 71 clinical samples) and correctly identified species and serotype in 100% and 84% of sequences, respectively. Known associations between specific serotypes and clinical syndromes are verified. Possible new associations are described to allow further independent testing.
Adenovirus Infections, Human | Adenoviruses, Human | Adolescent | Adult | Capsid Proteins | Child | Child, Preschool | DNA, Viral | Genome, Viral | Humans | Infant | Infant, Newborn | Middle Aged | Molecular Sequence Data | Phylogeny | Polymerase Chain Reaction | Serotyping | Sequence Analysis, DNA
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