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Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region

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URI: http://hdl.handle.net/20.500.12105/8278
ISSN: 0095-1137
DOI: 10.1128/JCM.43.12.6176-6182.2005
Publication date
2005-12
Authors
Jabado, O
Campos, R H
Rewers, M
Lipkin, W I
Palacios, G
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American Society for Microbiology (ASM)
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Abstract
Here we present a system for adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserved hexon gene fragment. The system was validated using 157 sequences (86 previously typed and 71 clinical samples) and correctly identified species and serotype in 100% and 84% of sequences, respectively. Known associations between specific serotypes and clinical syndromes are verified. Possible new associations are described to allow further independent testing.
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Adenovirus Infections, Human Adenoviruses, Human Adolescent Adult Capsid Proteins Child Child, Preschool DNA, Viral Genome, Viral Humans Infant Infant, Newborn Middle Aged Molecular Sequence Data Phylogeny Polymerase Chain Reaction Serotyping Sequence Analysis, DNA
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Bibliographic citation
J Clin Microbiol. 2005 Dec;43(12):6176-82.
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Centro Nacional de Microbiología (CNM)
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https://doi.org/10.1128/JCM.43.12.6176-6182.2005
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journal article