Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/7965
Pitfalls in spontaneous in vitro transformation of human mesenchymal stem cells
Exp Cell Res. 2010 May 15;316(9):1648-50.
Spontaneous in vitro transformation of human primary cells was, and continues to be, a scarcely described phenomenon. Only the description of the generation of the HaCAT cell line  is a canonical example, worldwide accepted. More recent examples included the emergence of tumorogenic populations upon in vitro culture of fetal human mesenchymal stem cells (hMSC), induced by GM-CSF and IL-4,  and bone marrow hMSC . Other examples have also been reported after very long-term in vitro culture of telomerized hMSCTerT . In this scenario, our previous results [5-7] were only a new observation to be added to this list. Only very recently similar results have been published , reporting spontaneous malignant transformation in 46 % of bone marrow–derived hMSC long-term cultures (5–106 weeks). However, other authors reported opposite results.
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