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dc.contributor.author | Mendez-Gonzalez, Diego | |
dc.contributor.author | Laurenti, Marco | |
dc.contributor.author | Latorre, Alfonso | |
dc.contributor.author | Somoza, Alvaro | |
dc.contributor.author | Vazquez, Ana | |
dc.contributor.author | Negredo, Anabel | |
dc.contributor.author | López-Cabarcos, Enrique | |
dc.contributor.author | Calderón, Oscar G. | |
dc.contributor.author | Melle, Sonia | |
dc.contributor.author | Rubio-Retama, Jorge | |
dc.date.accessioned | 2019-05-28T07:35:37Z | |
dc.date.available | 2019-05-28T07:35:37Z | |
dc.date.issued | 2017-03 | |
dc.identifier.citation | ACS Appl Mater Interfaces. 2017 Apr 12;9(14):12272-12281 | es_ES |
dc.identifier.issn | 1944-8244 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/7680 | |
dc.description.abstract | We present a sensor that exploits the phenomenon of upconversion luminescence to detect the presence of specific sequences of small oligonucleotides such as miRNAs among others. The sensor is based on NaYF4:Yb,Er@SiO2 nanoparticles functionalized with ssDNA that contain azide groups on the 3' ends. In the presence of a target sequence, interstrand ligation is possible via the click-reaction between one azide of the upconversion probe and a DBCO-ssDNA-biotin probe present in the solution. As a result of this specific and selective process, biotin is covalently attached to the surface of the upconversion nanoparticles. The presence of biotin on the surface of the nanoparticles allows their selective capture on a streptavidin-coated support, giving a luminescent signal proportional to the amount of target strands present in the test samples. With the aim of studying the analytical properties of the sensor, total RNA samples were extracted from healthy mosquitoes and were spiked-in with a specific target sequence at different concentrations. The result of these experiments revealed that the sensor was able to detect 10-17 moles per well (100 fM) of the target sequence in mixtures containing 100 ng of total RNA per well. A similar limit of detection was found for spiked human serum samples, demonstrating the suitability of the sensor for detecting specific sequences of small oligonucleotides under real conditions. In contrast, in the presence of noncomplementary sequences or sequences having mismatches, the luminescent signal was negligible or conspicuously reduced. | es_ES |
dc.description.sponsorship | The authors are grateful for the financial support from the Bill & Melinda Gates Foundation, with Grant OPP1128411, Asociación Española Contra el Cáncer, Santander-Universidad Complutense project PR26/16-12B-3, and from the Spanish MINECO for the projects MAT2014-55065-R, SAF2014-56763-R, and FIS2013-41709-P. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | ACS Publications | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject | DNA | es_ES |
dc.subject | Interstrand ligation | es_ES |
dc.subject | Nanoparticles | es_ES |
dc.subject | Sensor | es_ES |
dc.subject | Upconversion | es_ES |
dc.subject.mesh | DNA | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Luminescence | es_ES |
dc.subject.mesh | Oligonucleotides | es_ES |
dc.subject.mesh | Silicon Dioxide | es_ES |
dc.subject.mesh | Nanoparticles | es_ES |
dc.title | Oligonucleotide Sensor Based on Selective Capture of Upconversion Nanoparticles Triggered by Target-Induced DNA Interstrand Ligand Reaction | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución-NoComercial-CompartirIgual 4.0 Internacional | * |
dc.identifier.pubmedID | 28332400 | es_ES |
dc.format.volume | 9 | es_ES |
dc.format.number | 14 | es_ES |
dc.format.page | 12281 | es_ES |
dc.identifier.doi | 10.1021/acsami.7b00575 | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Bill & Melinda Gates Foundation | |
dc.contributor.funder | Asociación Española Contra el Cáncer | |
dc.contributor.funder | Banco Santander | |
dc.contributor.funder | Complutense University of Madrid (España) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1944-8252 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1021/acsami.7b00575 | es_ES |
dc.identifier.journal | ACS Applied Materials & Interfaces | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/MAT2014-55065-R | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2014-56763-R | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/FIS2013-41709-P | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/PR26/16-12B-3 | es_ES |
dc.rights.accessRights | open access | es_ES |