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dc.contributor.authorMendez-Gonzalez, Diego
dc.contributor.authorLaurenti, Marco
dc.contributor.authorLatorre, Alfonso
dc.contributor.authorSomoza, Alvaro
dc.contributor.authorVazquez, Ana 
dc.contributor.authorNegredo, Anabel 
dc.contributor.authorLópez-Cabarcos, Enrique
dc.contributor.authorCalderón, Oscar G.
dc.contributor.authorMelle, Sonia
dc.contributor.authorRubio-Retama, Jorge
dc.date.accessioned2019-05-28T07:35:37Z
dc.date.available2019-05-28T07:35:37Z
dc.date.issued2017-03
dc.identifier.citationACS Appl Mater Interfaces. 2017 Apr 12;9(14):12272-12281es_ES
dc.identifier.issn1944-8244es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7680
dc.description.abstractWe present a sensor that exploits the phenomenon of upconversion luminescence to detect the presence of specific sequences of small oligonucleotides such as miRNAs among others. The sensor is based on NaYF4:Yb,Er@SiO2 nanoparticles functionalized with ssDNA that contain azide groups on the 3' ends. In the presence of a target sequence, interstrand ligation is possible via the click-reaction between one azide of the upconversion probe and a DBCO-ssDNA-biotin probe present in the solution. As a result of this specific and selective process, biotin is covalently attached to the surface of the upconversion nanoparticles. The presence of biotin on the surface of the nanoparticles allows their selective capture on a streptavidin-coated support, giving a luminescent signal proportional to the amount of target strands present in the test samples. With the aim of studying the analytical properties of the sensor, total RNA samples were extracted from healthy mosquitoes and were spiked-in with a specific target sequence at different concentrations. The result of these experiments revealed that the sensor was able to detect 10-17 moles per well (100 fM) of the target sequence in mixtures containing 100 ng of total RNA per well. A similar limit of detection was found for spiked human serum samples, demonstrating the suitability of the sensor for detecting specific sequences of small oligonucleotides under real conditions. In contrast, in the presence of noncomplementary sequences or sequences having mismatches, the luminescent signal was negligible or conspicuously reduced.es_ES
dc.description.sponsorshipThe authors are grateful for the financial support from the Bill & Melinda Gates Foundation, with Grant OPP1128411, Asociación Española Contra el Cáncer, Santander-Universidad Complutense project PR26/16-12B-3, and from the Spanish MINECO for the projects MAT2014-55065-R, SAF2014-56763-R, and FIS2013-41709-P.es_ES
dc.language.isoenges_ES
dc.publisherACSes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectDNAes_ES
dc.subjectinterstrand ligationes_ES
dc.subjectnanoparticleses_ES
dc.subjectsensores_ES
dc.subjectupconversiones_ES
dc.subject.meshDNA es_ES
dc.subject.meshHumans es_ES
dc.subject.meshLuminescence es_ES
dc.subject.meshOligonucleotides es_ES
dc.subject.meshSilicon Dioxide es_ES
dc.subject.meshNanoparticles es_ES
dc.titleOligonucleotide Sensor Based on Selective Capture of Upconversion Nanoparticles Triggered by Target-Induced DNA Interstrand Ligand Reactiones_ES
dc.typeArtículoes_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.identifier.pubmedID28332400es_ES
dc.format.volume9es_ES
dc.format.number14es_ES
dc.format.page12281es_ES
dc.identifier.doi10.1021/acsami.7b00575es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderBill & Melinda Gates Foundationes_ES
dc.contributor.funderAsociación Española Contra el Cánceres_ES
dc.contributor.funderBanco Santanderes_ES
dc.contributor.funderUniversidad Complutense de Madrid (España)es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1944-8252es_ES
dc.relation.publisherversionhttps://doi.org/10.1021/acsami.7b00575es_ES
dc.identifier.journalACS Applied Materials & Interfaceses_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/MAT2014-55065-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2014-56763-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/FIS2013-41709-Pes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PR26/16-12B-3es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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Atribución-NoComercial-CompartirIgual 4.0 Internacional
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