dc.contributor.author | Seiringer, Peter | |
dc.contributor.author | Pritsch, Michael | |
dc.contributor.author | Flores-Chavez, Maria | |
dc.contributor.author | Marchisio, Edoardo | |
dc.contributor.author | Helfrich, Kerstin | |
dc.contributor.author | Mengele, Carolin | |
dc.contributor.author | Hohnerlein, Stefan | |
dc.contributor.author | Bretzel, Gisela | |
dc.contributor.author | Löscher, Thomas | |
dc.contributor.author | Hoelscher, Michael | |
dc.contributor.author | Berens-Riha, Nicole | |
dc.date.accessioned | 2019-02-13T12:35:21Z | |
dc.date.available | 2019-02-13T12:35:21Z | |
dc.date.issued | 2017-04-07 | |
dc.identifier.citation | Diagn Microbiol Infect Dis. 2017;88(3):225-232 | es_ES |
dc.identifier.issn | 07328893 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/7174 | |
dc.description.abstract | Due to increased migration, Chagas disease has become an international health problem. Reliable diagnosis of chronically infected people is crucial for prevention of non-vectorial transmission as well as treatment. This study compared four distinct PCR methods for detection of Trypanosoma cruzi DNA for the use in well-equipped routine diagnostic laboratories. DNA was extracted of T. cruzi-positive and negative patients' blood samples and cultured T. cruzi, T. rangeli as well as Leishmania spp. One conventional and two real-time PCR methods targeting a repetitive Sat-DNA sequence as well as one conventional PCR method targeting the variable region of the kDNA minicircle were compared for sensitivity, intra- and interassay precision, limit of detection, specificity and cross-reactivity. Considering the performance, costs and ease of use, an algorithm for PCR-diagnosis of patients with a positive serology for T. cruzi antibodies was developed. | es_ES |
dc.description.sponsorship | This research was supported by the German Center for InfectionResearch through the MD program (to MH, MP and PS). Overall,the project wasfinanced by the University of Munich (LMU). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Comparison | es_ES |
dc.subject | Conventional | es_ES |
dc.subject | Diagnosis | es_ES |
dc.subject | PCR | es_ES |
dc.subject | Real-time | es_ES |
dc.subject | Trypanosoma cruzi | es_ES |
dc.subject.mesh | Adolescent | es_ES |
dc.subject.mesh | Adult | es_ES |
dc.subject.mesh | Blood | es_ES |
dc.subject.mesh | Chagas Disease | es_ES |
dc.subject.mesh | Child, Preschool | es_ES |
dc.subject.mesh | Female | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Male | es_ES |
dc.subject.mesh | Middle Aged | es_ES |
dc.subject.mesh | Molecular Diagnostic Techniques | es_ES |
dc.subject.mesh | Polymerase Chain Reaction | es_ES |
dc.subject.mesh | Sensitivity and Specificity | es_ES |
dc.subject.mesh | Trypanosoma cruzi | es_ES |
dc.subject.mesh | Young Adult | es_ES |
dc.title | Comparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución-NoComercial-SinObraDerivada 4.0 Internacional | * |
dc.identifier.pubmedID | 28456430 | es_ES |
dc.format.volume | 88 | es_ES |
dc.format.number | 3 | es_ES |
dc.format.page | 232 | es_ES |
dc.identifier.doi | 10.1016/j.diagmicrobio.2017.04.003 | es_ES |
dc.contributor.funder | German Center for Infection Research (Alemania) | |
dc.contributor.funder | Ludwig-Maximilians-Universität München (Alemania) | |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1879-0070 | es_ES |
dc.relation.publisherversion | https://www.doi.org/10.1016/j.diagmicrobio.2017.04.003 | es_ES |
dc.identifier.journal | Diagnostic Microbiology and Infectious Disease | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |