Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/7174
Comparison of four PCR methods for efficient detection of Trypanosoma cruzi in routine diagnostics
Diagn Microbiol Infect Dis. 2017;88(3):225-232
Due to increased migration, Chagas disease has become an international health problem. Reliable diagnosis of chronically infected people is crucial for prevention of non-vectorial transmission as well as treatment. This study compared four distinct PCR methods for detection of Trypanosoma cruzi DNA for the use in well-equipped routine diagnostic laboratories. DNA was extracted of T. cruzi-positive and negative patients' blood samples and cultured T. cruzi, T. rangeli as well as Leishmania spp. One conventional and two real-time PCR methods targeting a repetitive Sat-DNA sequence as well as one conventional PCR method targeting the variable region of the kDNA minicircle were compared for sensitivity, intra- and interassay precision, limit of detection, specificity and cross-reactivity. Considering the performance, costs and ease of use, an algorithm for PCR-diagnosis of patients with a positive serology for T. cruzi antibodies was developed.
Adolescent | Adult | Blood | Chagas Disease | Child, Preschool | Female | Humans | Male | Middle Aged | Molecular Diagnostic Techniques | Polymerase Chain Reaction | Sensitivity and Specificity | Trypanosoma cruzi | Young Adult
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