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Título
Immunization with H1, HASPB1 and MML Leishmania proteins in a vaccine trial against experimental canine leishmaniasis
Autor(es)
Moreno, Javier ISCIII | Nieto Martinez, Francisco Javier ISCIII | Masina, S | Cañavate, Carmen ISCIII | Cruz, Israel ISCIII | Chicharro, Carmen ISCIII | Carrillo, Eugenia ISCIII | Napp, S | Reymond, C | Kaye, P M | Smith, D F | Fasel, N | Alvar, Jorge ISCIII
Fecha de publicación
2007-07-20
Cita
Vaccine. 2007; 20;25(29):5290-300.
Idioma
Inglés
Tipo de documento
journal article
Resumen
The protective capabilities of three Leishmania recombinant proteins - histone 1 (H1) and hydrophilic acylated surface protein B1 (HASPB1) immunized singly, or together as a protein cocktail vaccine with Montanide, and the polyprotein MML immunized with MPL-SE adjuvant - were assessed in beagle dogs. Clinical examination of the dogs was carried out periodically under blinded conditions and the condition of the dogs defined as asymptomatic or symptomatic. At the end of the trial, we were able to confirm that following infection with L. infantum promastigotes, five out of eight dogs immunized with H1 Montanide, and four out of eight dogs immunized with either the combination of HASPB1 with Montanide or the combination of H1+HASPB1 with Montanidetrade mark, remained free of clinical signs, compared with two out of seven dogs immunized with the polyprotein MML and adjuvant MPL-SE, and two out of eight dogs in the control group. The results demonstrate that HASPB1 and H1 antigens in combination with Montanide were able to induce partial protection against canine leishmaniasis, even under extreme experimental challenge conditions.
Palabras clave
MESH
Adjuvants, Immunologic | Animals | Antibodies, Protozoan | Blood Chemical Analysis | Body Weight | Cell Proliferation | Dog Diseases | Dogs | Enzyme-Linked Immunosorbent Assay | Female | Leishmania | Leishmaniasis | Leukocytes, Mononuclear | Male | Protozoan Vaccines | Vaccines, Subunit | Vaccines, Synthetic
Versión en línea
DOI
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