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dc.contributor.authorAguinagalde, Leire 
dc.contributor.authorCorsini, Bruno 
dc.contributor.authorDomenech, Arnau
dc.contributor.authorDomenech Lucas, Mirian 
dc.contributor.authorCámara, Jordi
dc.contributor.authorArdanuy, Carmen
dc.contributor.authorGarcía, Ernesto
dc.contributor.authorLiñares, Josefina
dc.contributor.authorFenoll, Asuncion 
dc.contributor.authorYuste, Jose Enrique 
dc.date.accessioned2019-01-10T11:53:10Z
dc.date.available2019-01-10T11:53:10Z
dc.date.issued2015
dc.identifier.citationPLoS One. 2015 Sep 14;10(9):e0137565es_ES
dc.identifier.issn1932-6203es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/6988
dc.description.abstractCapsular switching allows pre-existing clones of Streptococcus pneumoniae expressing vaccine serotypes to escape the vaccine-induced immunity by acquisition of capsular genes from pneumococci of a non-vaccine serotype. Here, we have analysed the clonal composition of 492 clinical isolates of serotype 11A causing invasive disease in Spain (2000-2012), and their ability to evade the host immune response. Antibiograms, serotyping and molecular typing were performed. The restriction profiles of pbp2x, pbp1a and pbp2b genes were also analysed. Interaction with the complement components C1q, C3b, C4BP, and factor H was explored whereas opsonophagocytosis assays were performed using a human cell line differentiated to neutrophils. Biofilm formation and the polymorphisms of the major autolysin LytA were evaluated. The main genotypes of the 11A pneumococci were: ST62 (447 isolates, 90.6%), followed by ST6521 (35 isolates, 7.3%) and ST838 (10 isolates, 2.1%). Beta lactam resistant serotype 11A variants of genotypes ST838 and ST6521 closely related to the Spain9V-ST156 clone were first detected in 2005. A different pattern of evasion of complement immunity and phagocytosis was observed between genotypes. The emergence of one vaccine escape variant of Spain9V-ST156 (ST652111A), showing a high potential to avoid the host immune response, was observed. In addition, isolates of ST652111A showed higher ability to produce biofilms than ST83811A or ST6211A, which may have contributed to the emergence of this PEN-resistant ST652111A genotype in the last few years. The emergence of penicillin-resistant 11A invasive variants of the highly successful ST156 clonal complex merits close monitoring.es_ES
dc.description.sponsorshipThis work was supported by grants SAF2012-39444-C01/02 from Ministerio de Economía y Competitividad (MINECO) to EG and JY and PI11/00763 from Fondo de Investigaciones Sanitarias de la Seguridad Social to CA and JL. Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES) is an initiative of ISCIII. LA and BC were supported, respectively, by an FPI fellowship from MINECO and a fellowship from the Brazilian Program Ciência Sem Fronteiras (CsF) from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Science (PLOS) es_ES
dc.type.hasVersionVoRes_ES
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAdolescent es_ES
dc.subject.meshAdult es_ES
dc.subject.meshChild es_ES
dc.subject.meshChild, Preschool es_ES
dc.subject.meshFemale es_ES
dc.subject.meshGene Expression Regulation, Bacterial es_ES
dc.subject.meshGenotype es_ES
dc.subject.meshHL-60 Cells es_ES
dc.subject.meshHistory, Ancient es_ES
dc.subject.meshHumans es_ES
dc.subject.meshMale es_ES
dc.subject.meshPneumococcal Vaccines es_ES
dc.subject.meshSpain es_ES
dc.subject.meshAmoxicillin es_ES
dc.subject.meshBacterial Proteins es_ES
dc.subject.meshDrug Resistance, Bacterial es_ES
dc.subject.meshImmune Evasion es_ES
dc.subject.meshSerogroup es_ES
dc.subject.meshStreptococcus pneumoniae es_ES
dc.titleEmergence of Amoxicillin-Resistant Variants of Spain9V-ST156 Pneumococci Expressing Serotype 11A Correlates with Their Ability to Evade the Host Immune Responsees_ES
dc.typejournal articlees_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID26368279es_ES
dc.format.volume10es_ES
dc.format.number9es_ES
dc.format.pagee0137565es_ES
dc.identifier.doi10.1371/journal.pone.0137565es_ES
dc.description.peerreviewedes_ES
dc.identifier.e-issn1932-6203es_ES
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0137565es_ES
dc.identifier.journalPloS onees_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2012-39444-C01/02es_ES
dc.rights.accessRightsopen accesses_ES


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Atribución 4.0 Internacional
Este Item está sujeto a una licencia Creative Commons: Atribución 4.0 Internacional