dc.contributor.author | Aguinagalde, Leire | |
dc.contributor.author | Corsini, Bruno | |
dc.contributor.author | Domenech, Arnau | |
dc.contributor.author | Domenech Lucas, Mirian | |
dc.contributor.author | Cámara, Jordi | |
dc.contributor.author | Ardanuy, Carmen | |
dc.contributor.author | García, Ernesto | |
dc.contributor.author | Liñares, Josefina | |
dc.contributor.author | Fenoll, Asuncion | |
dc.contributor.author | Yuste, Jose Enrique | |
dc.date.accessioned | 2019-01-10T11:53:10Z | |
dc.date.available | 2019-01-10T11:53:10Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | PLoS One. 2015 Sep 14;10(9):e0137565 | es_ES |
dc.identifier.issn | 1932-6203 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/6988 | |
dc.description.abstract | Capsular switching allows pre-existing clones of Streptococcus pneumoniae expressing vaccine serotypes to escape the vaccine-induced immunity by acquisition of capsular genes from pneumococci of a non-vaccine serotype. Here, we have analysed the clonal composition of 492 clinical isolates of serotype 11A causing invasive disease in Spain (2000-2012), and their ability to evade the host immune response. Antibiograms, serotyping and molecular typing were performed. The restriction profiles of pbp2x, pbp1a and pbp2b genes were also analysed. Interaction with the complement components C1q, C3b, C4BP, and factor H was explored whereas opsonophagocytosis assays were performed using a human cell line differentiated to neutrophils. Biofilm formation and the polymorphisms of the major autolysin LytA were evaluated. The main genotypes of the 11A pneumococci were: ST62 (447 isolates, 90.6%), followed by ST6521 (35 isolates, 7.3%) and ST838 (10 isolates, 2.1%). Beta lactam resistant serotype 11A variants of genotypes ST838 and ST6521 closely related to the Spain9V-ST156 clone were first detected in 2005. A different pattern of evasion of complement immunity and phagocytosis was observed between genotypes. The emergence of one vaccine escape variant of Spain9V-ST156 (ST652111A), showing a high potential to avoid the host immune response, was observed. In addition, isolates of ST652111A showed higher ability to produce biofilms than ST83811A or ST6211A, which may have contributed to the emergence of this PEN-resistant ST652111A genotype in the last few years. The emergence of penicillin-resistant 11A invasive variants of the highly successful ST156 clonal complex merits close monitoring. | es_ES |
dc.description.sponsorship | This work was supported by grants SAF2012-39444-C01/02 from Ministerio de Economía y Competitividad (MINECO) to EG and JY and PI11/00763 from Fondo de Investigaciones Sanitarias de la Seguridad Social to CA and JL. Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES) is an initiative of ISCIII. LA and BC were supported, respectively, by an FPI fellowship from MINECO and a fellowship from the Brazilian Program Ciência Sem Fronteiras (CsF) from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science (PLOS) | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.mesh | Adolescent | es_ES |
dc.subject.mesh | Adult | es_ES |
dc.subject.mesh | Child | es_ES |
dc.subject.mesh | Child, Preschool | es_ES |
dc.subject.mesh | Female | es_ES |
dc.subject.mesh | Gene Expression Regulation, Bacterial | es_ES |
dc.subject.mesh | Genotype | es_ES |
dc.subject.mesh | HL-60 Cells | es_ES |
dc.subject.mesh | History, Ancient | es_ES |
dc.subject.mesh | Humans | es_ES |
dc.subject.mesh | Male | es_ES |
dc.subject.mesh | Pneumococcal Vaccines | es_ES |
dc.subject.mesh | Spain | es_ES |
dc.subject.mesh | Amoxicillin | es_ES |
dc.subject.mesh | Bacterial Proteins | es_ES |
dc.subject.mesh | Drug Resistance, Bacterial | es_ES |
dc.subject.mesh | Immune Evasion | es_ES |
dc.subject.mesh | Serogroup | es_ES |
dc.subject.mesh | Streptococcus pneumoniae | es_ES |
dc.title | Emergence of Amoxicillin-Resistant Variants of Spain9V-ST156 Pneumococci Expressing Serotype 11A Correlates with Their Ability to Evade the Host Immune Response | es_ES |
dc.type | journal article | es_ES |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 26368279 | es_ES |
dc.format.volume | 10 | es_ES |
dc.format.number | 9 | es_ES |
dc.format.page | e0137565 | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0137565 | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.identifier.e-issn | 1932-6203 | es_ES |
dc.relation.publisherversion | https://doi.org/10.1371/journal.pone.0137565 | es_ES |
dc.identifier.journal | PloS one | es_ES |
dc.repisalud.centro | ISCIII::Centro Nacional de Microbiología | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2012-39444-C01/02 | es_ES |
dc.rights.accessRights | open access | es_ES |