Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/5161
Title
Kalirin and CHD7: novel endothelial dysfunction indicators in circulating extracellular vesicles from hypertensive patients with albuminuria
Author(s)
de la Cuesta, Fernando | Baldan-Martin, Montserrat | Moreno-Luna, Rafael | Alvarez-Llamas, Gloria | Gonzalez-Calero, Laura | Mourino-Alvarez, Laura | Sastre-Oliva, Tamara | Lopez, Juan Antonio CNIC | Vazquez, Jesus CNIC | Ruiz-Hurtado, Gema | Segura, Julian | Vivanco, Fernando | Ruilope, Luis M | Barderas, Maria G
Date issued
2017
Citation
Oncotarget. 2017; 8(9):15553-15562
Language
Inglés
Document type
journal article
Abstract
Despite of the great advances in anti-hypertensive therapies, many patients under Renin-Angiotensin-System (RAS) suppression develop albuminuria, which is a clear indicator of therapeutic inefficiency. Hence, indicators of vascular function are needed to assess patients' condition and help deciding future therapies. Proteomic analysis of circulating extracellular vesicles (EVs) showed two proteins, kalirin and chromodomain-helicase-DNA-binding protein 7 (CHD7), increased in albuminuric patients. A positive correlation of both with the expression of the endothelial activation marker E-selectin was found in EVs. In vitro analysis using TNFa-treated adult human endothelial cells proved their involvement in endothelial cell activation. Hence, we propose protein levels of kalirin and CHD7 in circulating EVs as novel endothelial dysfunction markers to monitor vascular condition in hypertensive patients with albuminuria.
Subject
Extracellular vesicles | Proteomics | Endothelial dysfunction | Hypertension | Albuminuria | ANGIOTENSIN SYSTEM SUPPRESSION | OXIDATIVE STRESS | CELLS | MICROALBUMINURIA | DISEASE
Online version
DOI
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