Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/5111
miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p
Gutierrez-Vazquez, Cristina CNIC | Rodriguez-Galan, Ana CNIC | Fernandez-Alfara, Marcos CNIC | Mittelbrunn, Maria CNIC | Sanchez-Cabo, Fatima CNIC | Jorge Martinez-Herrera, Dannys | Ramirez-Huesca, Marta CNIC | Pascual-Montano, Alberto | Sanchez-Madrid, Francisco CNIC
Sci Rep. 2017; 7(1):3508
microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3'UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells.
MESSENGER-RNA | MICRORNA | EXPRESSION | DIFFERENTIATION | PI3K | PREDICTION | P85-ALPHA | DUPLEXES | DICER