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dc.contributor.author | Gutierrez-Vazquez, Cristina | |
dc.contributor.author | Rodriguez-Galan, Ana | |
dc.contributor.author | Fernandez-Alfara, Marcos | |
dc.contributor.author | Mittelbrunn, Maria | |
dc.contributor.author | Sanchez-Cabo, Fatima | |
dc.contributor.author | Jorge Martinez-Herrera, Dannys | |
dc.contributor.author | Ramirez-Huesca, Marta | |
dc.contributor.author | Pascual-Montano, Alberto | |
dc.contributor.author | Sanchez-Madrid, Francisco | |
dc.date.accessioned | 2017-10-20T10:23:11Z | |
dc.date.available | 2017-10-20T10:23:11Z | |
dc.date.issued | 2017 | |
dc.identifier | ISI:000403318400062 | |
dc.identifier.citation | Sci Rep. 2017; 7(1):3508 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/5111 | |
dc.description.abstract | microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3'UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells. | |
dc.description.sponsorship | We thank Miguel Vicente-Manzanares for help with English editing and Almudena R. Ramiro for helpful discussions. We appreciate help from Gloria Martinez del Hoyo on DCs experiments set up. We also thank the CNIC Genomics, Bioinformatics and Cellomics Units for technical support. This work was supported by grants SAF2014-55579R from Ministerio de Economia y Competitividad-Spain, ERC-2011-AdG 294340-GENTRIS, CIBER CARDIOVASCULAR (FEDER and Instituto de Salud Carlos III), PIE-13-00041 and INDISNET S2011-BMD-2332 (F.S.M.). The Centro Nacional de Investigaciones Cardiovasculares (CNIC, Spain) is supported by the Ministerio de Economia y Competitividad-Spain and the Pro-CNIC Foundation. | |
dc.language.iso | eng | |
dc.publisher | Nature Publishing Group | |
dc.type.hasVersion | VoR | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | MESSENGER-RNA | |
dc.subject | MICRORNA | |
dc.subject | EXPRESSION | |
dc.subject | DIFFERENTIATION | |
dc.subject | PI3K | |
dc.subject | PREDICTION | |
dc.subject | P85-ALPHA | |
dc.subject | DUPLEXES | |
dc.subject | DICER | |
dc.title | miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p | |
dc.type | journal article | |
dc.rights.license | Atribución 4.0 Internacional | * |
dc.identifier.pubmedID | 28615644 | |
dc.format.volume | 7 | |
dc.identifier.doi | https://doi.org/10.1038/s41598-017-03689-7 | |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | |
dc.contributor.funder | Instituto de Salud Carlos III | |
dc.contributor.funder | Fundación ProCNIC | |
dc.description.peerreviewed | Sí | |
dc.relation.publisherversion | https://doi.org/10.1038/s41598-017-03689-7 | |
dc.identifier.journal | Scientific Report | |
dc.repisalud.orgCNIC | CNIC::Grupos de investigación::Comunicación Intercelular en la Respuesta Inflamatoria | |
dc.repisalud.orgCNIC | CNIC::Unidades técnicas::Bioinformática | |
dc.repisalud.institucion | CNIC | |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/FP7/294340/EU | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/SAF2014-55579R | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ES/PIE-13-00041 | es_ES |
dc.rights.accessRights | open access | es_ES |