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dc.contributor.authorGutierrez-Vazquez, Cristina 
dc.contributor.authorRodriguez-Galan, Ana 
dc.contributor.authorFernandez-Alfara, Marcos 
dc.contributor.authorMittelbrunn, Maria 
dc.contributor.authorSanchez-Cabo, Fatima 
dc.contributor.authorJorge Martinez-Herrera, Dannys
dc.contributor.authorRamirez-Huesca, Marta 
dc.contributor.authorPascual-Montano, Alberto
dc.contributor.authorSanchez-Madrid, Francisco 
dc.date.accessioned2017-10-20T10:23:11Z
dc.date.available2017-10-20T10:23:11Z
dc.date.issued2017
dc.identifierISI:000403318400062
dc.identifier.citationSci Rep. 2017; 7(1):3508
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/20.500.12105/5111
dc.description.abstractmicroRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3'UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells.
dc.description.sponsorshipWe thank Miguel Vicente-Manzanares for help with English editing and Almudena R. Ramiro for helpful discussions. We appreciate help from Gloria Martinez del Hoyo on DCs experiments set up. We also thank the CNIC Genomics, Bioinformatics and Cellomics Units for technical support. This work was supported by grants SAF2014-55579R from Ministerio de Economia y Competitividad-Spain, ERC-2011-AdG 294340-GENTRIS, CIBER CARDIOVASCULAR (FEDER and Instituto de Salud Carlos III), PIE-13-00041 and INDISNET S2011-BMD-2332 (F.S.M.). The Centro Nacional de Investigaciones Cardiovasculares (CNIC, Spain) is supported by the Ministerio de Economia y Competitividad-Spain and the Pro-CNIC Foundation.
dc.language.isoeng
dc.publisherNature Publishing Group 
dc.type.hasVersionVoR
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectMESSENGER-RNA
dc.subjectMICRORNA
dc.subjectEXPRESSION
dc.subjectDIFFERENTIATION
dc.subjectPI3K
dc.subjectPREDICTION
dc.subjectP85-ALPHA
dc.subjectDUPLEXES
dc.subjectDICER
dc.titlemiRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p
dc.typejournal article
dc.rights.licenseAtribución 4.0 Internacional*
dc.identifier.pubmedID28615644
dc.format.volume7
dc.identifier.doihttps://doi.org/10.1038/s41598-017-03689-7
dc.contributor.funderMinisterio de Economía y Competitividad (España) 
dc.contributor.funderInstituto de Salud Carlos III 
dc.contributor.funderFundación ProCNIC 
dc.description.peerreviewed
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-017-03689-7
dc.identifier.journalScientific Report
dc.repisalud.orgCNICCNIC::Grupos de investigación::Comunicación Intercelular en la Respuesta Inflamatoria
dc.repisalud.orgCNICCNIC::Unidades técnicas::Bioinformática
dc.repisalud.institucionCNIC
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/294340/EUes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2014-55579Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PIE-13-00041es_ES
dc.rights.accessRightsopen accesses_ES


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