2024-03-29T04:43:56Zhttp://repisalud.isciii.es/oai/requestoai:repisalud.isciii.es:20.500.12105/51112022-10-14T13:09:47Zcom_20.500.12105_2145com_20.500.12105_2051com_20.500.12105_2144com_20.500.12105_2152col_20.500.12105_2146col_20.500.12105_2153
Repisalud
author
Gutierrez-Vazquez, Cristina
author
Rodriguez-Galan, Ana
author
Fernandez-Alfara, Marcos
author
Mittelbrunn, Maria
author
Sanchez-Cabo, Fatima
author
Jorge Martinez-Herrera, Dannys
author
Ramirez-Huesca, Marta
author
Pascual-Montano, Alberto
author
Sanchez-Madrid, Francisco
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Fundación ProCNIC
2017-10-20T10:23:11Z
2017-10-20T10:23:11Z
2017
Sci Rep. 2017; 7(1):3508
2045-2322
http://hdl.handle.net/20.500.12105/5111
28615644
https://doi.org/10.1038/s41598-017-03689-7
Scientific Report
microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3'UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells.
eng
MESSENGER-RNA
MICRORNA
EXPRESSION
DIFFERENTIATION
PI3K
PREDICTION
P85-ALPHA
DUPLEXES
DICER
miRNA profiling during antigen-dependent T cell activation: A role for miR-132-3p
journal article
URL
https://repisalud.isciii.es/bitstream/20.500.12105/5111/1/miRNAProfilingDuringAntigen-dependent_2017
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miRNAProfilingDuringAntigen-dependent_2017
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https://repisalud.isciii.es/bitstream/20.500.12105/5111/2/miRNAProfilingDuringAntigen-dependent_2017_Supp
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miRNAProfilingDuringAntigen-dependent_2017_Supp
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https://repisalud.isciii.es/bitstream/20.500.12105/5111/19/miRNAProfilingDuringAntigen-dependent_2017.txt
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35231
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miRNAProfilingDuringAntigen-dependent_2017.txt
URL
https://repisalud.isciii.es/bitstream/20.500.12105/5111/21/miRNAProfilingDuringAntigen-dependent_2017_Supp.txt
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MD5
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miRNAProfilingDuringAntigen-dependent_2017_Supp.txt
URL
https://repisalud.isciii.es/bitstream/20.500.12105/5111/31/miRNAProfilingDuringAntigen-dependent_2017.txt
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miRNAProfilingDuringAntigen-dependent_2017.txt
URL
https://repisalud.isciii.es/bitstream/20.500.12105/5111/33/miRNAProfilingDuringAntigen-dependent_2017_Supp.txt
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MD5
243f9c014b25266508b1c88730a62c3e
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miRNAProfilingDuringAntigen-dependent_2017_Supp.txt