Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/20163
Título
Haemophilus influenzae induces steroid-resistant inflammatory responses in COPD
Autor(es)
Fecha de publicación
2015-12-07
Cita
Cosio BG, Jahn A, Iglesias A, Shafiek H, Busquets X, Agusti García-Navarro A. Haemophilus influenzae induces steroid-resistant inflammatory responses in COPD. BMC Pulm Med. 2015 Dec 07;15:157.
Idioma
Inglés
Tipo de documento
research article
Resumen
Background: Chronic obstructive pulmonary disease (COPD) is an inflammatory disorder partially resistant to glucocorticoids. A reduced histone deacetylase (HDAC) activity has been proposed to explain this resistance. Haemophilus influenzae frequently colonizes the airways of COPD patients, where it enhances inflammation. The effects of Haemophilus influenzae on HDAC activity have not been investigated before. Methods: The effects of the presence or absence of Haemophilus influenzae ex-vivo and in vitro were studied. To this end, we determined: (1) cytokine release in alveolar macrophages (AM) from 7 patients with COPD, 5 healthy smokers, 6 healthy non-smokers and (2) HDAC activity, nuclear factor kappa B (NF-kappa B) activation in a macrophage-like cell line (PMA-transformed U937 cells) co-cultured with epithelial cells. Experiments were repeated with dexamethasone (1 mu M) and/or the HDAC enhancer theophylline (10 mu M). Results: Haemophilus influenzae induced a steroid-resistant inflammatory response in AM from COPD and controls and decreased HDAC activity, activated NF-kappa B and induced the secretion of several cytokines (IL-6, IL-8, IL-1 beta, IL-10 and TNF-alpha) (p < 0.001 for all comparisons) in the macrophage-like cell line. Dexamethasone reduced NF-kappa B activation but it did not modify HDAC activity. The addition of theophylline to dexamethasone increased HDAC activity and suppressed cytokine release completely, without modifying NF-kappa B activation. Conclusions: These results indicate that Haemophilus influenzae reduces HDAC activity and induces a NF-kappa B mediated inflammatory response that is only partially suppressed by glucocorticoids irrespective of having COPD. Yet, the latter can be fully restored by targeting HDAC activity.
Palabras clave
COPD exacerbation | Glucocorticoids | Colonization | Histone deacetylase | Nuclear Factor-kappa B | Theophylline
MESH
Glucocorticoids | Aged | Case-Control Studies | Blotting, Western | Adult | In Vitro Techniques | Pulmonary Disease, Chronic Obstructive | Humans | Smoking | Histone Deacetylases | Inflammation | Theophylline | Cell Line | Bronchodilator Agents | Middle Aged | Male | Cytokines | NF-kappa B | Female | Haemophilus Infections | Haemophilus influenzae | Macrophages, Alveolar | Respiratory Tract Infections | Dexamethasone
DECS
Dexametasona | Macrófagos Alveolares | Infecciones por Haemophilus | Citocinas | FN-kappa B | Femenino | Haemophilus influenzae | Técnicas In Vitro | Línea Celular | Masculino | Teofilina | Histona Desacetilasas | Fumar | Enfermedad Pulmonar Obstructiva Crónica | Broncodilatadores | Humanos | Persona de Mediana Edad | Inflamación | Anciano | Adulto | Western Blotting | Estudios de Casos y Controles | Glucocorticoides | Infecciones del Sistema Respiratorio
Versión en línea
DOI
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