Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/20159
Título
Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cells
Autor(es)
Fecha de publicación
2015-11-12
Cita
Gomez-Florit M, Monjo M, Ramis JM. Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cells. Sci Rep. 2015 Nov 12;5:16593.
Idioma
Inglés
Tipo de documento
research article
Resumen
Periodontal disease (PD) is the result of an infection and chronic inflammation of the gingiva that may lead to its destruction and, in severe cases, alveolar bone and tooth loss. The ultimate goal of periodontal treatment is to achieve periodontal soft and hard tissues regeneration. We previously selected quercitrin, a catechol-containing flavonoid, as a potential agent for periodontal applications. In this study, we tested the ability of quercitrin to alter biomarker production involved in periodontal regeneration on primary human gingival fibroblasts (hGF) and primary human mesenchymal stem cells (hMSC) cultured under basal and inflammatory conditions. To mimic PD inflammatory status, interleukin-1 beta (IL-1 beta) was used. The expression of different genes related to inflammation and extracellular matrix were evaluated and prostaglandin E2 (PGE2) production was quantified in hGFs; alkaline phosphatase (ALP) activity and calcium content were analysed in hMSCs. Quercitrin decreased the release of the inflammatory mediator PGE2 and partially re-established the impaired collagen metabolism induced by IL-1 beta treatment in hGFs. Quercitrin also increased ALP activity and mineralization in hMSCs, thus, it increased hMSCs differentiation towards the osteoblastic lineage. These findings suggest quercitrin as a novel bioactive molecule with application to enhance both soft and hard tissue regeneration of the periodontium.
MESH
Young Adult | Adult | Fibroblasts | Cell Differentiation | Gene Expression Regulation | Humans | Cells, Cultured | Periodontium | Gingiva | Middle Aged | Male | Osteoblasts | Regeneration | Cytokines | Inflammation Mediators | Interleukin-1beta | Female | Osteogenesis | Quercetin
DECS
Mediadores de Inflamación | Citocinas | Femenino | Encía | Periodoncio | Masculino | Regulación de la Expresión Génica | Osteoblastos | Diferenciación Celular | Humanos | Persona de Mediana Edad | Adulto Joven | Células Cultivadas | Fibroblastos | Regeneración | Quercetina | Osteogénesis | Adulto | Interleucina-1beta
Versión en línea
DOI
Aparece en las colecciones
Acceso a texto completo