Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/20143
Title
Effect of consuming a grape seed supplement with abundant phenolic compounds on the oxidative status of healthy human volunteers
Author(s)
Date issued
2015-09-09
Citation
Grases F, Prieto RM, Fernandez-Cabot RA, Costa-Bauza A, Sanchez AM, Prodanov M. Effect of consuming a grape seed supplement with abundant phenolic compounds on the oxidative status of healthy human volunteers. Nutr J. 2015 Sep 09;14:94.
Language
Inglés
Document type
research article
Abstract
Background: Diverse enzymatic and non-enzymatic antioxidants provide protection against reactive oxygen species in humans and other organisms. The nonenzymatic antioxidants include low molecular mass molecules such as plant-derived phenols. Aim of study: This study identified the major phenolic compounds of a grape seed extract by HPLC and analyzed the effect of consumption of biscuits enriched with this extract on the urinary oxidative status of healthy subjects by measurement of urine redox potential. Methods: The major phenolic compounds were characterized in a red grape seed extract separated by HPLC with detection by a photodiode array (PDA), fluorescence (FL) and quadrupole mass spectrometer (MS). A nutritional study in a healthy volunteers group was done. Each volunteer ate eight traditional biscuits with no red grape seed extract supplementation. The second day each volunteer ate eight traditional biscuits supplemented with 0.6 % (wt/wt) of grape seed extract. An overnight urine sample was obtained for each treatment. The redox potential was measured at 25 degrees C using a potentiometer in each urine sample. Results: Epicatechin, catechin, procyanidin dimers B1 to B4, and the procyanidin trimer C2 were the major phenolic components in the extract. Epicatechin gallate and procyanidin dimers B1-3-G and B2-3'-G were the major galloylated flavan-3-ols. The forty-six healthy volunteers each shown a reduction of the urine redox potential after the treatment by traditional biscuits supplemented with the grape seed extract. Conclusions: This simple dietary intervention significantly reduced (33 %) the urine redox potential, reflecting an overall increase in antioxidant status. Incorporation of plant-derived phenols in the diet may increase anti-oxidative status.
MESH
Antioxidants | Oxidative Stress | Young Adult | Adult | Reactive Oxygen Species | Humans | Biflavonoids | Chromatography, High Pressure Liquid | Healthy Volunteers | Adolescent | Proanthocyanidins | Vitis | Middle Aged | Catechin | Male | Seeds | Female | Phenols | Grape Seed Extract | Dietary Supplements
DECS
Biflavonoides | Proantocianidinas | Femenino | Catequina | Voluntarios Sanos | Adolescente | Masculino | Semillas | Fenoles | Cromatografía Líquida de Alta Presión | Humanos | Persona de Mediana Edad | Vitis | Extracto de Semillas de Uva | Adulto Joven | Especies Reactivas de Oxígeno | Estrés Oxidativo | Antioxidantes | Adulto | Suplementos Dietéticos
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