Please use this identifier to cite or link to this item:http://hdl.handle.net/20.500.12105/20105
Title
Blood cell transcriptomic-based early biomarkers of adverse programming effects of gestational calorie restriction and their reversibility by leptin supplementation
Author(s)
Date issued
2015-03-13
Citation
Konieczna J, Sánchez J, Palou March M, Pico C, Palou Oliver A. Blood cell transcriptomic-based early biomarkers of adverse programming effects of gestational calorie restriction and their reversibility by leptin supplementation. Sci Rep. 2015 Mar 13;5:9088.
Language
Inglés
Document type
research article
Abstract
The challenge of preventing major chronic diseases requires reliable, early biomarkers. Gestational mild undernutrition in rats is enough to program the offspring to develop later pathologies; the intake of leptin, a breastmilk component, during lactation may reverse these programming effects. We used these models to identify, in peripheral blood mononuclear cells (PBMCs), transcriptomic-based early biomarkers of programmed susceptibility to later disorders, and explored their response to neonatal leptin intake. Microarray analysis was performed in PBMCs from the offspring of control and 20% gestational calorie-restricted dams (CR), and CR-rats supplemented with physiological doses of leptin throughout lactation. Notably, leptin supplementation normalised 218 of the 224 mRNA-levels identified in PBMCs associated to undernutrition during pregnancy. These markers may be useful for early identification and subsequent monitoring of individuals who are at risk of later diseases and would specifically benefit from the intake of appropriate amounts of leptin during lactation.
MESH
Animals, Newborn | Cluster Analysis | Body Weights and Measures | Gene Expression Regulation | Blood Cells | Leukocytes, Mononuclear | Pregnancy | Transcriptome | Male | Biomarkers | Leptin | Female | Gene Expression Profiling | Rats | Animals | Caloric Restriction | Dietary Supplements | Reproducibility of Results | Genome-Wide Association Study
DECS
Animales | Reproducibilidad de los Resultados | Biomarcadores | Femenino | Masculino | Restricción Calórica | Regulación de la Expresión Génica | Estudio de Asociación del Genoma Completo | Células Sanguíneas | Ratas | Leucocitos Mononucleares | Embarazo | Animales Recién Nacidos | Suplementos Dietéticos | Leptina | Perfilación de la Expresión Génica | Pesos y Medidas Corporales | Análisis por Conglomerados | Transcriptoma
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