Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/19922
Título
Distribution of Aspergillus species and prevalence of azole resistance in clinical and environmental samples from a Spanish hospital during a three-year study period
Autor(es)
Lucio, Jose ISCIII | Alcazar-Fuoli, Laura ISCIII | Gil, Horacio ISCIII | Cano-Pascual, Samuel | Hernandez-Egido, Sara | Cuetara, Maria Soledad | Mellado, Emilia ISCIII
Fecha de publicación
2024-04
Cita
Mycoses. 2024 Apr;67(4):e13719.
Idioma
Inglés
Tipo de documento
research article
Resumen
Background: Surveillance studies are crucial for updating trends in Aspergillus species and antifungal susceptibility information. Objectives: Determine the Aspergillus species distribution and azole resistance prevalence during this 3-year prospective surveillance study in a Spanish hospital. Materials and methods: Three hundred thirty-five Aspergillus spp. clinical and environmental isolates were collected during a 3-year study. All isolates were screened for azole resistance using an agar-based screening method and resistance was confirmed by EUCAST antifungal susceptibility testing. The azole resistance mechanism was confirmed by sequencing the cyp51A gene and its promoter. All Aspergillus fumigatus strains were genotyped using TRESPERG analysis. Results: Aspergillus fumigatus was the predominant species recovered with a total of 174 strains (51.94%). The rest of Aspergillus spp. were less frequent: Aspergillus niger (14.93%), Aspergillus terreus (9.55%), Aspergillus flavus (8.36%), Aspergillus nidulans (5.37%) and Aspergillus lentulus (3.28%), among other Aspergillus species (6.57%). TRESPERG analysis showed 99 different genotypes, with 72.73% of the strains being represented as a single genotype. Some genotypes were common among clinical and environmental A. fumigatus azole-susceptible strains, even when isolated months apart. We describe the occurrence of two azole-resistant A. fumigatus strains, one clinical and another environmental, that were genotypically different and did not share genotypes with any of the azole-susceptible strains. Conclusions: Aspergillus fumigatus strains showed a very diverse population although several genotypes were shared among clinical and environmental strains. The isolation of azole-resistant strains from both settings suggest that an efficient analysis of clinical and environmental sources must be done to detect azole resistance in A. fumigatus.
Palabras clave
MESH
Aspergillosis | Aspergillus nidulans | Humans | Azoles | Antifungal Agents | Prevalence | Prospective Studies | Drug Resistance, Fungal | Aspergillus fumigatus | Hospitals | Fungal Proteins | Microbial Sensitivity Tests
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