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dc.contributor.author | González-Sastre, Rosa | |
dc.contributor.author | Coronel Lopez, Raquel | |
dc.contributor.author | Bernabeu-Zornoza, Adela | |
dc.contributor.author | Mateos-Martínez, Patricia | |
dc.contributor.author | Rosca, Andreea | |
dc.contributor.author | López-Alonso, Victoria | |
dc.contributor.author | Liste-Noya, Isabel | |
dc.date.accessioned | 2024-03-21T14:27:31Z | |
dc.date.available | 2024-03-21T14:27:31Z | |
dc.date.issued | 2024-02 | |
dc.identifier.citation | J Tissue Eng. 2024 Feb 9:15:20417314231226027. | es_ES |
dc.identifier.issn | 2041-7314 | es_ES |
dc.identifier.uri | http://hdl.handle.net/20.500.12105/19036 | |
dc.description.abstract | Human cerebral organoids (hCOs) offer the possibility of deepening the knowledge of human brain development, as well as the pathologies that affect it. The method developed here describes the efficient generation of hCOs by going directly from two-dimensional (2D) pluripotent stem cell (PSC) cultures to three-dimensional (3D) neuroepithelial tissue, avoiding dissociation and aggregation steps. This has been achieved by subjecting 2D cultures, from the beginning of the neural induction step, to dual-SMAD inhibition in combination with CHIR99021. This is a simple and reproducible protocol in which the hCOs generated develop properly presenting proliferative ventricular zones (VZs) formed by neural precursor and radial glia (RG) that differentiate to give rise to mature neurons and glial cells. The hCOs present additional cell types such as oligodendrocyte precursors, astrocytes, microglia-like cells, and endothelial-like cells. This new approach could help to overcome some of the existing limitations in the field of organoid biotechnology, facilitating its execution in any laboratory setting. | es_ES |
dc.description.sponsorship | The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Grant PID2021-126715OB-I00 funded by MCIN/AEI/10.13039/501100011033 and “ERDF A way of making Europe,” by the Grant of Instituto de Salud Carlos III (ISCIII) PI22CIII/00055, grant RTI2018-101663-B-100 funded by MCIN/AEI/ and the UFIECPY 398/19, PEJ2018-004965 grant to RGS funded by AEI. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | SAGE Publishing | es_ES |
dc.type.hasVersion | VoR | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | * |
dc.subject | Human cerebral organoid | es_ES |
dc.subject | Pluripotent stem cells | es_ES |
dc.subject | Embryonic stem cells | es_ES |
dc.subject | Neuroepithelium | es_ES |
dc.subject | Ventricular zones | es_ES |
dc.subject | Embryoid body | es_ES |
dc.title | Efficient generation of human cerebral organoids directly from adherent cultures of pluripotent stem cells | es_ES |
dc.type | research article | es_ES |
dc.rights.license | Atribución-NoComercial 4.0 Internacional | * |
dc.identifier.pubmedID | 38343770 | es_ES |
dc.format.volume | 15 | es_ES |
dc.format.page | 20417314231226027 | es_ES |
dc.identifier.doi | 10.1177/20417314231226027 | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación (España) | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación (España) | es_ES |
dc.contributor.funder | Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF) | es_ES |
dc.description.peerreviewed | Sí | es_ES |
dc.relation.publisherversion | https://doi.org/10.1177/20417314231226027 | es_ES |
dc.identifier.journal | Journal of tissue engineering | es_ES |
dc.repisalud.centro | ISCIII::Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC) | es_ES |
dc.repisalud.institucion | ISCIII | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PEJ2018-004965 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/UFIECPY398/19 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/RTI2018-101663-B-100 | es_ES |
dc.relation.projectFECYT | info:eu-repo/grantAgreement/ES/PID2021-126715OB-I00 | es_ES |
dc.relation.projectFIS | info:fis/Instituto de Salud Carlos III///PI22-ISCIII Proyectos de I+D+I en salud (AES 2022).Intramurales (2022)/PI22CIII/00055 | es_ES |