Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/19036
Título
Efficient generation of human cerebral organoids directly from adherent cultures of pluripotent stem cells
Autor(es)
González-Sastre, Rosa ISCIII | Coronel Lopez, Raquel ISCIII | Bernabeu-Zornoza, Adela ISCIII | Mateos-Martínez, Patricia ISCIII | Rosca, Andreea ISCIII | López-Alonso, Victoria | Liste-Noya, Isabel ISCIII
Fecha de publicación
2024-02
Cita
J Tissue Eng. 2024 Feb 9:15:20417314231226027.
Idioma
Inglés
Tipo de documento
research article
Resumen
Human cerebral organoids (hCOs) offer the possibility of deepening the knowledge of human brain development, as well as the pathologies that affect it. The method developed here describes the efficient generation of hCOs by going directly from two-dimensional (2D) pluripotent stem cell (PSC) cultures to three-dimensional (3D) neuroepithelial tissue, avoiding dissociation and aggregation steps. This has been achieved by subjecting 2D cultures, from the beginning of the neural induction step, to dual-SMAD inhibition in combination with CHIR99021. This is a simple and reproducible protocol in which the hCOs generated develop properly presenting proliferative ventricular zones (VZs) formed by neural precursor and radial glia (RG) that differentiate to give rise to mature neurons and glial cells. The hCOs present additional cell types such as oligodendrocyte precursors, astrocytes, microglia-like cells, and endothelial-like cells. This new approach could help to overcome some of the existing limitations in the field of organoid biotechnology, facilitating its execution in any laboratory setting.
Palabras clave
Human cerebral organoid | Pluripotent stem cells | Embryonic stem cells | Neuroepithelium | Ventricular zones | Embryoid body
Versión en línea
DOI
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