Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/17468
Título
Proteomics Analysis Reveals That Structural Proteins of the Virion Core and Involved in Gene Expression Are the Main Source for HLA Class II Ligands in Vaccinia Virus-Infected Cells
Autor(es)
Lorente, Elena ISCIII | Martin-Galiano, Antonio Javier ISCIII | Barnea, Eilon | Barriga, Alejandro ISCIII | Palomo-Sanz, Concepcion ISCIII | García-Arriaza, Juan | Mir-Gerrero, Carmen ISCIII | Lauzurica, Pilar ISCIII | Esteban, Mariano | Admon, Arie | Lopez, Daniel ISCIII
Fecha de publicación
2019-03-01
Cita
J Proteome Res. 2019 Mar 1;18(3):900-911.
Idioma
Inglés
Tipo de documento
research article
Resumen
Protective cellular and humoral immune responses require previous recognition of viral antigenic peptides complexed with human leukocyte antigen (HLA) class II molecules on the surface of the antigen presenting cells. The HLA class II-restricted immune response is important for the control and the clearance of poxvirus infection including vaccinia virus (VACV), the vaccine used in the worldwide eradication of smallpox. In this study, a mass spectrometry analysis was used to identify VACV ligands bound to HLA-DR and -DP class II molecules present on the surface of VACV-infected cells. Twenty-six naturally processed viral ligands among the tens of thousands of cell peptides bound to HLA class II proteins were identified. These viral ligands arose from 19 parental VACV proteins: A4, A5, A18, A35, A38, B5, B13, D1, D5, D7, D12, D13, E3, E8, H5, I2, I3, J2, and K2. The majority of these VACV proteins yielded one HLA ligand and were generated mainly, but not exclusively, by the classical HLA class II antigen processing pathway. Medium-sized and abundant proteins from the virion core and/or involved in the viral gene expression were the major source of VACV ligands bound to HLA-DR and -DP class II molecules. These findings will help to understand the effectiveness of current poxvirus-based vaccines and will be important in the design of new ones.
Palabras clave
Antigen processing | Bioinformatics tools | Cellular immune response | HLA | Immunoproteomics | Mass spectrometry | Peptides | T cells | Vaccine | Virus
MESH
Ligands | Viral Structural Proteins | Cells, Cultured | Gene Expression | Histocompatibility Antigens Class II | Humans | Mass Spectrometry | Poxviridae | Proteomics | Vaccinia | Vaccinia virus | Viral Proteins | Viral Vaccines | Virion
Versión en línea
DOI
Aparece en las colecciones