Por favor, use este identificador para citar o enlazar este Item:http://hdl.handle.net/20.500.12105/17439
Título
Tubulin homolog TubZ in a phage-encoded partition system
Autor(es)
Fecha de publicación
2012-05-15
Cita
Proc Natl Acad Sci USA. 2012 May 15;109(20):7711-6.
Idioma
Inglés
Tipo de documento
research article
Resumen
Partition systems are responsible for the process whereby large and essential plasmids are accurately positioned to daughter cells during bacterial division. They are typically made of three components: a centromere-like DNA zone, an adaptor protein, and an assembling protein that is either a Walker-box ATPase (type I) or an actin-like ATPase (type II). A recently described type III segregation system has a tubulin/FtsZ-like protein, called TubZ, for plasmid movement. Here, we present the 2.3 Å structure and dynamic assembly of a TubZ tubulin homolog from a bacteriophage and unravel the Clostridium botulinum phage c-st type III partition system. Using biochemical and biophysical approaches, we prove that a gene upstream from tubZ encodes the partner TubR and localize the centromeric region (tubS), both of which are essential for anchoring phage DNA to the motile TubZ filaments. Finally, we describe a conserved fourth component, TubY, which modulates the TubZ-R-S complex interaction.
Palabras clave
MESH
Models, Molecular | Amino Acid Sequence | Bacterial Proteins | Bacteriophages | Base Sequence | Biophysics | Centromere | Cloning, Molecular | Clostridium botulinum | Cluster Analysis | Computational Biology | Crystallization | Cytokinesis | Molecular Sequence Data | Multiprotein Complexes | Phylogeny | Plasmids | Sequence Analysis, DNA | Tubulin
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